Nutrigenetic evaluation of constitutive androstane receptor (CAR, NR1i3) on feed efficiency in Nelore cattle

• Grant number: 10/05650-5
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2010
• End date: May 31, 2011
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Genetics and Improvement of Domestic Animals
• Principal Investigator: Heidge Fukumasu
• Grantee: Heidge Fukumasu
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated researchers: Flávio Vieira Meirelles ; José Bento Sterman Ferraz ; Paulo Roberto Leme ; Rodrigo da Costa Gomes ; Saulo da Luz e Silva

Abstract

In the last years, the focus of animal nutrition research shifted from epidemiology and physiology to Molecular Biology and Genetics. This occurred because it is impossible to understand the effects of nutrients in health and disease without a profound comprehension of how the gene-nutrient (or vice-versa) interacts from the molecular point of view. There are several molecular pathways regulated by specific transcriptional factors that mediated these above mentioned interactions by up or down-regulating gene expression of numerous genes which possess specific promoter regions. One interesting transcriptional factor is the Constitutive Androstane Receptor (CAR, NR1i3), recently been demonstrated as a central regulator of energetic metabolism, decreasing gluconeogenesis and lipogenesis. Recently, it was demonstrated that CAR is an anti-obesity nuclear receptor that improves insulin sensitivity. Then, it could be speculated that genetic variation in the DNA sequence of CAR gene may be responsible for characteristics of economical relevance in beef cattle, specially feed efficiency. Then, the aim of this research project will be evaluate the presence of Single Nucleotide Polymorphisms (SNPs) in the whole extension of the CAR gene in Nellore cattle regarding feed efficiency. To achieve this, blood will be collected from 50 male animals previously evaluated regarding their feed efficiency. DNA will be extracted and the entire sequence of the CAR gene will be amplified by the Polymerase chain reaction with 8 different primer pairs (to cover all the gene sequence of CAR). Then, these PCR products will be sequenced and aligned to find possible new SNPs. If novel SNPs were found, they will be characterized in silico for their possible changes in the structure of CAR protein. At last, the SNPs will be statistically correlated to some phenotypic characteristics referent to feed efficiency. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FUKUMASU, HEIDGE; ROCHETTI, ARINA L.; PIRES, PEDRO R. L.; SILVA, EDSON R.; MESQUITA, LIGIA G.; STREFEZZI, RICARDO F.; DE CARVALHO, DANIEL D.; DAGLI, MARIA L.. Constitutive Androstane Receptor Ligands Modulate the Anti-Tumor Efficacy of Paclitaxel in Non-Small Cell Lung Cancer Cells. PLoS One, v. 9, n. 6, JUN 24 2014. (10/05650-5, 09/11081-6)

FUKUMASU, H.; CORDEIRO, Y. G.; ROCHETTI, A. L.; BARRA, C. N.; SAMORA, T. S.; STREFEZZI, R. F.; DAGLI, M. L. Z.. Expression of NR1I3 in mouse lung tumors induced by the tobacco-specific nitrosamine 4-(methylnitrosamino)-4-(3-pyridyl)-1-butanone. Brazilian Journal of Medical and Biological Research, v. 48, n. 3, p. 240-244, MAR 2015. (10/05650-5, 09/11081-6)

ALEXANDRE, PAMELA A.; GOMES, RODRIGO C.; SANTANA, MIGUEL H. A.; SILVA, SAULO L.; LEME, PAULO R.; MUDADU, MAURICIO A.; REGITANO, LUCIANA C. A.; MEIRELLES, FLVIO V.; FERRAZ, JOSE B. S.; FUKUMASU, HEIDGE. Bovine NR1I3 gene polymorphisms and its association with feed efficiency traits in Nellore cattle. META GENE, v. 2, p. 12-pg., 2014-12-01. (08/11363-9, 10/05650-5)

MACEDO MOTA, LUCIO FLAVIO; BONAFE, CRISTINA MOREIRA; ALEXANDRE, PAMELA ALMEIDA; SANTANA, MIGUEL HENRIQUE; NOVAIS, FRANCISCO JOSE; TORIYAMA, ERIKA; PIRES, ALDRIN VIEIRA; SILVA, SAULO DA LUZ; LEME, PAULO ROBERTO; STERMAN FERRAZ, JOSE BENTO; et al. Circulating leptin and its muscle gene expression in Nellore cattle with divergent feed efficiency. JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY, v. 8, SEP 1 2017. (14/02493-7, 10/05650-5, 14/07566-2)

MACEDO MOTA, LUCIO FLAVIO; BONAFE, CRISTINA MOREIRA; ALEXANDRE, PAMELA ALMEIDA; SANTANA, MIGUEL HENRIQUE; NOVAIS, FRANCISCO JOSE; TORIYAMA, ERIKA; PIRES, ALDRIN VIEIRA; SILVA, SAULO DA LUZ; LEME, PAULO ROBERTO; STERMAN FERRAZ, JOSE BENTO; et al. Circulating leptin and its muscle gene expression in Nellore cattle with divergent feed efficiency. JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY, v. 8, p. 5-pg., 2017-09-01. (14/07566-2, 10/05650-5, 14/02493-7)