Utilization of laser Er-Cr: YSGG in soft and hard tissue repair and in the treatment of induced periodontal disease: analyses histological and immune-inflammatory in rats

• Grant number: 09/00479-9
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2009
• End date: June 30, 2011
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Rosemary Adriana Chierici Marcantonio
• Grantee: Rosemary Adriana Chierici Marcantonio
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Laser, or Light Amplification by Stimulated Emission of Radiation, is a non-ionizant radiation that exhibits some specific characteristics such as monocromatocity, coherency and collimation. In dentistry, they are separated into two groups: low and high-level laser equipments. Low-level lasers are used for bacterial reduction and healing stimulation. High-level lasers have photothermic and photomechanical interaction with tissues, creating incisions of irradiated tissues and calculus removal from the tooth surface. Among these, Er-Cr:YSGG laser (Erbium-Chromium: Yttrium, Scandium, Gallium , Garnet) appears as a new advent in periodontal therapy and brings out many applications possibilities for soft and hard tissues and periodontal treatment. Periodontal studies mostly involve scaling and root planning procedures, morphologic evaluation on dentin, as adjunct of conventional therapy, comparison with ultrasonic instruments and hard tissue effects. The aim of this work is to evaluate the tissue repair after incision by Er-Cr:YSGG laser and your influence in the treatment of induced periodontal disease. Histological and immunological (RANKL and OPG protein expression) analysis will be performed after incisions by Er-Cr:YSGG irradiation in soft and hard tissue. Male rats, mean weight 200-250g, will be used in this study. At baseline, they will receive two incisions on dorsum, one with scalpel and the other with Er-Cr:YSGG laser (2W power output); two incisions in calvarias, one with burr and another with Er-Cr:YSGG laser (3W power output) and one incision in each tibia, where which tibia will receive one of the treatments, burr or Er-Cr:YSGG (3W power output). Side distribution will be randomly made and incisions done by a single operator. The animals will be distributed into 4 groups corresponding to the experimental periods: 3, 7, 15, and 30 post-operative days, representing the sacrifice data. In the treatment of induced periodontal disease histological and immune-inflammatory analyses will be done. Sixty rats will be used and one maxillary first molar of each animal will be assigned randomly to receive the cotton ligature in a submarginal position to induce experimental periodontitis during seven days. After this period, the animals will be randomly distributed in four groups and evaluated in three different experimental periods (7, 15 and 30 days). Group I: None treatment will be done, Group II: conventional scaling and root planning, Group III: application of Er,Cr:YSGG laser and Group IV: Er,Cr:YSGG laser therapy in association with scaling and root planning. After each experimental period the animals will be sacrificed for laboratorial procedures and subsequent histological and immune-inflammatory analyses. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PAVONE, CHAINE; PERUSSI, LIVIA RODRIGUES; PIMENTEL LOPES DE OLIVEIRA, GUILHERME JOSE; SCARDUELI, CASSIO ROCHA; CIRELLI, JONI AUGUSTO; CERRI, PAULO SERGIO; MARCANTONIO JUNIOR, ELCIO; SPOLIDORIO, LUIS CARLOS; CHIERICI MARCANTONIO, ROSEMARY ADRIANA. Effect of Er,Cr:YSGG laser application in the treatment of experimental periodontitis. Lasers in Medical Science, v. 30, n. 3, p. 993-999, APR 2015. (09/00479-9)