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Gene expression in sites with chronic periodontitis in diabetic individuals

Grant number: 08/04280-0
Support type:Regular Research Grants
Duration: June 01, 2009 - May 31, 2011
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Poliana Mendes Duarte
Grantee:Poliana Mendes Duarte
Home Institution: Universidade de Guarulhos (UNG). Campus Guarulhos-Centro. Guarulhos , SP, Brazil

Abstract

This study will evaluate the gene expression of inflammatory factors (Toll like receptor [TLR]-2 e TLR-4, tumor necrosis factor (TNF)-alpha, interleukin [IL]-17, interferon [INF]-gamma, IL-10, IL-4, receptor activator of NF-kappaB ligand [RANKL], osteoprotegerin [OPG]) in sites with chronic periodontitis in diabetic individuals. Sixty gingival biopsies will be harvested and divided into four groups: Sites periodontally healthy in individuals systemically healthy (n=15); Sites with chronic periodontitis in systemically healthy subjects (n=15); Sites with chronic periodontitis in controlled diabetic subjects (n=15); Sites with chronic periodontitis in uncontrolled diabetic subjects (n=15). The biopsies will be evaluate for RNAm expression of TLR-2, TLR-4, TNF-alpha, IL-17, INF-gamma, IL-10, IL-4, RANKL e OPG by quantitative PCR. mRNA levels data will be averaged and an intergroup comparison will be performed by one way-ANOVA. (AU)

Scientific publications (9)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BASTOS, M. F.; TUCCI, M. A.; DE SIQUEIRA, A.; DE FAVERI, M.; FIGUEIREDO, L. C.; VALLIM, P. C.; DUARTE, P. M. Diabetes may affect the expression of matrix metalloproteinases and their inhibitors more than smoking in chronic periodontitis. JOURNAL OF PERIODONTAL RESEARCH, v. 52, n. 2, p. 292-299, APR 2017. Web of Science Citations: 12.
DUARTE, POLIANA MENDES; MIRANDA, TAMIRES SZEREMESKE; LIMA, JADSON ALMEIDA; DIAS GONCALVES, TIAGO EDUARDO; SANTOS, VANESSA RENATA; BASTOS, MARTA FERREIRA; RIBEIRO, FERNANDA VIEIRA. Expression of Immune-Inflammatory Markers in Sites of Chronic Periodontitis in Patients With Type 2 Diabetes. Journal of Periodontology, v. 83, n. 4, p. 426-434, APR 2012. Web of Science Citations: 36.
MENDONCA, ADRIANA CUTRIM; SANTOS, VANESSA RENATA; RIBEIRO, FERNANDA VIEIRA; LIMA, JADSON ALMEIDA; MIRANDA, TAMIRES SZEREMESKE; FERES, MAGDA; DUARTE, POLIANA MENDES. Surgical and non-surgical therapy with systemic antimicrobials for residual pockets in type 2 diabetics with chronic periodontitis: a pilot study. Journal of Clinical Periodontology, v. 39, n. 4, p. 368-376, APR 2012. Web of Science Citations: 9.
RIBEIRO, F. V.; SANTOS, V. R.; BASTOS, M. F.; DE MIRANDA, T. S.; VIEIRA, A. R.; DE FIGUEIREDO, L. C.; DUARTE, P. M. A preliminary study on the FAM5C expression in generalized chronic periodontitis. ORAL DISEASES, v. 18, n. 2, p. 147-152, MAR 2012. Web of Science Citations: 7.
SANTOS, VANESSA RENATA; LIMA, JADSON ALMEIDA; MIRANDA, TAMIRES SZEREMESKE; FERES, MAGDA; ZIMMERMANN, GLAUCIA SANTOS; NOGUEIRA-FILHO, GETULIO DA ROCHA; DUARTE, POLIANA MENDES. Relationship between glycemic subsets and generalized chronic periodontitis in type 2 diabetic Brazilian subjects. ARCHIVES OF ORAL BIOLOGY, v. 57, n. 3, p. 293-299, MAR 2012. Web of Science Citations: 9.
DUARTE, POLIANA M.; NAPIMOGA, MARCELO H.; FAGNANI, ELLEN C.; SANTOS, VANESSA R.; BASTOS, MARTA F.; RIBEIRO, FERNANCLA V.; ARAUJO, VERA C.; DEMASI, ANA PAULA D. The expression of antioxidant enzymes in the gingivae of type 2 diabetics with chronic periodontitis. ARCHIVES OF ORAL BIOLOGY, v. 57, n. 2, p. 161-168, FEB 2012. Web of Science Citations: 8.
SANTOS, V. R.; RIBEIRO, F. V.; LIMA, J. A.; MIRANDA, T. S.; FERES, M.; BASTOS, M. F.; DUARTE, P. M. Partial- and full-mouth scaling and root planing in type 2 diabetic subjects: a 12-mo follow-up of clinical parameters and levels of cytokines and osteoclastogenesis-related factors. JOURNAL OF PERIODONTAL RESEARCH, v. 47, n. 1, p. 45-54, FEB 2012. Web of Science Citations: 16.
NAPIMOGA, M. H.; NUNES, L. H. A. C.; MACIEL, A. A. B.; DEMASI, A. P. D.; BENATTI, B. B.; SANTOS, V. R.; BASTOS, M. F.; DE MIRANDA, T. S.; DUARTE, P. M. Possible Involvement of IL-21 and IL-10 on Salivary IgA Levels in Chronic Periodontitis Subjects. Scandinavian Journal of Immunology, v. 74, n. 6, p. 596-602, DEC 2011. Web of Science Citations: 9.
RIBEIRO, FERNANDA VIEIRA; DE MENDONCA, ADRIANA CUTRIM; SANTOS, VANESSA RENATA; BASTOS, MARTA FERREIRA; FIGUEIREDO, LUCIENE CRISTINA; DUARTE, POLIANA MENDES. Cytokines and Bone-Related Factors in Systemically Healthy Patients With Chronic Periodontitis and Patients With Type 2 Diabetes and Chronic Periodontitis. Journal of Periodontology, v. 82, n. 8, p. 1187-1196, AUG 2011. Web of Science Citations: 53.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.