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Isolation, some semi-synthetic obtainment and biological activity evaluation of the some lignans isolated from dry seeds of Piper cubeba

Grant number:08/02717-1
Support Opportunities:Regular Research Grants
Start date: June 01, 2008
End date: May 31, 2010
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Márcio Luís Andrade e Silva
Grantee:Márcio Luís Andrade e Silva
Host Institution: Reitoria. Universidade de Franca (UNIFRAN). Franca , SP, Brazil
City of the host institution:Franca

Abstract

Dibenzylbutyrolactone lignans show many biological activities, including antitumor, anti-inflammatory, trypanocidal, and other. Among these lignans, the (-)-cubebin, which has anti-inflammatory activity, is present in seeds of Piper cubeba (Piperaceae). The aim of this project is the extraction, isolation and the purification of the lignans cubebinolide (19), cubebinone (20), iatein (21), cubebinine (23), clusin (24), isoatein (26) and axantine (29) (Figura 1), from the dry seeds of P. cubeba, as well as the obtainment of their semi-synthetic derivatives (41)-(44) (Esquema 1), (45)-(48) (Esquema 2) (49)-(56) (Esquema 3), (57)-(59) (Esquema 4) and (60)-(63) (Esquema 5). At the beginning, 1 kg of seeds will be dried and, after that, submitted to extraction, by percolation, using ethanol. The obtained crude ethanolic extract will be sequentially partitioned with hexane and ethyl acetate. The ethyl acetate fraction will be submitted to several chromatographic processes in order to isolate compounds (19)-(21), (23), (24), (26) e (29). The obtained compounds will be modified and all obtained compounds will be submitted to biological evaluation. (AU)

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Scientific publications
(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)
TIMPLE, JULIE MARIE V.; MAGALHAES, LIZANDRA GUIDI; SOUZA REZENDE, KAREN CRISTINA; PEREIRA, ANA CAROLINA; CUNHA, WILSON ROBERTO; ANDRADE E SILVA, MARCIO LUIS; MORTENSEN, OLE VALENTE; FONTANA, ANDREIA C. K.. The Lignan (-)-Hinokinin Displays Modulatory Effects on Human Monoamine and GABA Transporter Activities. Journal of Natural Products, v. 76, n. 10, p. 1889-1895, . (08/02717-1, 09/15207-4)