Diagnosis of visceral leishmaniasis in peripheral blood samples using conventional microbiologic and kDNA- based techniques

• Grant number: 10/50304-8
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2010
• End date: January 31, 2013
• Field of knowledge: Biological Sciences - Parasitology
• Principal Investigator: Lúcia Maria Almeida Braz
• Grantee: Lúcia Maria Almeida Braz
• Host Institution: Instituto de Medicina Tropical de São Paulo (IMT). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Although known as a rural endemic, the American Visceral Leishmaniasis (LVA) presented a large modification in its epidemiologic behaviour. Detected in several Brazilian states at the end of the 1980s, the urbanization is the main of them and another is the geographical expansion, associated to a epidemic behavior. Until 1999 São Paulo State had not registered autóctones cases, as an example of this phenomenon. Since that year 1542 patients acquired LVA in São Paulo State (Amato et al., 2009). Before of this LVA urbanization, is emergent the improvement and availability of validated diagnosis techniques, including the molecular tools which could substitute invasive techniques. By this study we intend assess the following visceral leishmaniasis diagnosis techniques, microscopic examination (Giemsa or Panóptico stained smears), culture (NNN/BHI) and kDNA using peripheral blood and bone marrow aspirate samples as templates. We are looking for a minimally invasive and easily performed representing by the peripheral blood techniques which could appear after the assessment of its sensitivity in relationship with the bone marrow. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BIANCONCINI TRINDADE, MARIA ANGELA; DA CRUZ SILVA, LANA LUIZA; ALMEIDA BRAZ, LUCIA MARIA; AMATO, VALDIR SABBAGA; NAAFS, BERNARD; SOTTO, MIRIAN NACAGAMI. Post-kala-azar dermal leishmaniasis and leprosy: case report and literature review. BMC INFECTIOUS DISEASES, v. 15, NOV 23 2015. (10/50304-8)

NATALIA SOUZA DE, DE GODOY; VERA DEMARCHI, AIELLO; REGINA MAIA, DE SOUZA; THELMA, OKAY; MARIA ALMEIDA, BRAZ LUCIA. Unusual Clinical Manifestations of Leishmania (L.) infantum chagasi in an HIV-coinfected Patient and the Relevance of ITS1-PCR-RFLP: A Case Report. IRANIAN JOURNAL OF PARASITOLOGY, v. 13, n. 4, p. 655-660, OCT-DEC 2018. (10/50304-8)