Structural characterization of enzymes from antibiotic biosynthetic pathways with biotechnological interest

• Grant number: 10/15971-3
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: September 01, 2011
• End date: August 31, 2016
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Marcio Vinicius Bertacine Dias
• Grantee: Marcio Vinicius Bertacine Dias
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant(s): 14/50324-0 - Towards safer aminogrycoside antibiotics: structure, function and engineering studies of key biosynthetic enzymes, AP.R
• Associated scholarship(s): 14/24486-2 - Structural basis of sensibility of Mycobacterium tuberculosis and M. leprae dihydropteroate synthase to the sulfonamide drugs, BP.MS; 14/07843-6 - Structural analysis of key enzymes for gentamicin and sisomicin biosynthesis, BP.DD; 13/26242-0 - Design of new antibiotics based in the fragment based drug discovery technique from the inhibition of vitamin biosynthesis in Mycobacterium tuberculosis, BP.PD; + associated scholarships 13/15906-5 - Fragment based drug discovery (FBDD) applied to two enzymes of folate biosynthetic pathway from Mycobacterium tuberculosis, BP.DR; 12/23427-7 - Structural analysis of halogenases identified in biosynthetic gene clusters of glycopeptide antibiotics., BP.MS; 12/16631-7 - Obtainment and crystallization of glycosyltransferases of biotechnological interest of the macrolide antibiotic biosynthesis, BP.IC; 11/13686-2 - Structural characterization of enzymes from antibiotic biosynthetic pathways with biotechnological interest, BP.JP - associated scholarships

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BERTACINE DIAS, MARCIO V.; SANTOS, JADEMILSON C.; LIBREROS-ZUNIGA, GERARDO A.; RIBEIRO, JOAO A.; CHAVEZ-PACHECO, SAIR M.. Folate biosynthesis pathway: mechanisms and insights into drug design for infectious diseases. Future Medicinal Chemistry, v. 10, n. 8, p. 935-959, APR 2018. (15/09188-8, 13/15906-5, 10/15971-3)

LUHAVAYA, HANNA; DIAS, MARCIO V. B.; WILLIAMS, SIMON R.; HONG, HUI; DE OLIVEIRA, LUCIANA G.; LEADLAY, PETER F.. Enzymology of Pyran RingA Formation in Salinomycin Biosynthesis. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, v. 54, n. 46, p. 13622-13625, NOV 9 2015. (10/15971-3, 11/17510-6)

BERTACINE DIAS, MARCIO VINICIUS; TYRAKIS, PETROS; DOMINGUES, ROMENIA RAMOS; PAES LEME, ADRIANA FRANCO; BLUNDELL, TOM L.. Mycobacterium tuberculosis Dihydrofolate Reductase Reveals Two Conformational States and a Possible Low Affinity Mechanism to Antifolate Drugs. Structure, v. 22, n. 1, p. 94-103, JAN 7 2014. (10/15971-3)

DE ARAUJO, NATALIA CERRONE; BURY, PRISCILA DOS SANTOS; TAVARES, MAURICIO TEMOTHEO; HUANG, FANGLU; PARISE-FILHO, ROBERTO; LEADLAY, PETER; BERTACINE DIAS, MARCIO VINICIUS. Crystal Structure of GenD2, an NAD-Dependent Oxidoreductase Involved in the Biosynthesis of Gentamicin. ACS Chemical Biology, v. 14, n. 5, p. 925-933, MAY 2019. (10/15971-3, 15/09188-8, 14/07843-6, 14/50324-0)

BURY, PRISCILA DOS SANTOS; HUANG, FANGLU; LI, SICONG; SUN, YUHUI; LEADLAY, PETER F.; BERTACINE DIAS, MARCIO VINICIUS. Structural Basis of the Selectivity of GenN, an Aminoglycoside N-Methyltransferase Involved in Gentamicin Biosynthesis. ACS Chemical Biology, v. 12, n. 11, p. 2779-2787, NOV 2017. (14/07843-6, 10/15971-3, 15/09188-8, 14/50324-0)

WILSON, CAROLINA; DE OLIVEIRA, GABRIEL S.; ADRIANI, PATRICIA P.; CHAMBERGO, FELIPE S.; DIAS, MARCIO V. B.. Structure of a soluble epoxide hydrolase identified in Trichoderma reesei. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1865, n. 8, p. 1039-1045, AUG 2017. (15/09188-8, 10/15971-3, 14/24107-1, 15/03329-9)

CARDOSO, TABATA P.; DE SA, LARISSA A.; BURY, PRISCILA DOS S.; CHAVEZ-PACHECO, SAIR M.; DIAS, MARCIO V. B.. Cloning, expression, purification and biophysical analysis of two putative halogenases from the glycopeptide A47,934 gene cluster of Streptomyces toyocaensis. Protein Expression and Purification, v. 132, p. 9-18, APR 2017. (10/15971-3, 12/23427-7, 12/16631-7)

LIBREROS-ZUNIGA, GERARDO ANDRES; SILVA, CATHARINA DOS SANTOS; FERREIRA, RAFAELA SALGADO; BERTACINE DIAS, MARCIO VINICIUS. Structural Basis for the Interaction and Processing of beta-Lactam Antibiotics by L,D-Transpeptidase 3 (Ldt(Mt3)) from Mycobacterium tuberculosis. ACS INFECTIOUS DISEASES, v. 5, n. 2, p. 260-271, FEB 2019. (15/09188-8, 10/15971-3, 16/18721-4)

RIBEIRO, JOAO A.; HAMMER, ALEXANDER; LIBREROS-ZUNIGA, GERARDO A.; CHAVEZ-PACHECO, SAIR M.; TYRAKIS, PETROS; DE OLIVEIRA, GABRIEL S.; KIRKMAN, TIMOTHY; EL BAKALI, JAMAL; ROCCO, SILVANA A.; SFORCA, MAURICIO L.; et al. Using a Fragment-Based Approach to Identify Alternative Chemical Scaffolds Targeting Dihydrofolate Reductase from Mycobacterium tuberculosis. ACS INFECTIOUS DISEASES, v. 6, n. 8, p. 2192-2201, AUG 14 2020. (10/15971-3, 13/15906-5, 18/00351-1)

LI, SICONG; BURY, PRISCILA DOS SANTOS; HUANG, FANGLU; GUO, JUNHONG; SUN, GUO; REVA, ANNA; HUANG, CHUAN; JIAN, XINYUN; LI, YUAN; ZHOU, JIAHAI; et al. Mechanistic Insights into Dideoxygenation in Gentamicin Biosynthesis. ACS CATALYSIS, v. 11, n. 19, p. 12274-12283, OCT 1 2021. (14/07843-6, 15/09188-8, 10/15971-3, 18/00351-1)

TORMET-GONZALEZ, GABRIELA D.; WILSON, CAROLINA; DE OLIVEIRA, GABRIEL STEPHANI; DOS SANTOS, JADEMILSON CELESTINO; DE OLIVEIRA, LUCIANA G.; DIAS, MARCIO VINICIUS BERTACINE. An epoxide hydrolase from endophytic Streptomycess hows unique structural features and wide biocatalytic activity. ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, v. 76, p. 8-pg., 2020-09-01. (19/10564-5, 18/00351-1, 15/09188-8, 11/06209-3, 14/50249-8, 10/15971-3, 13/26242-0, 14/12727-5)