Gametes and artificial fertilization of surubim-do-paraíba, Steindachneridion parahybae, an endangered species

• Grant number: 11/02818-5
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2011
• End date: November 30, 2013
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Elizabeth Romagosa
• Grantee: Elizabeth Romagosa
• Host Institution: Instituto de Pesca. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: Danilo Caneppele ; Maria Ines Borella

Abstract

The Paraíba do Sul River Basin is located in the region with the highest level of urban and industrial development in the country, geografically isolated from the other basins. It has been suffering the impact of anthropic activity, threatening with extinction a noble endemic fish species, the "surubim-do-Paraiba", Steindachneridion parahybae. The impact of the environmental degradation originated in the beginning of human occupation, as well as with the construction of dams for the generation of electricity and/or water uptake, without a proper conservation management. Because it is critically endangered, only a complete change in this reality will make the preservation of freshwater ecosystems possible, recovering and protecting the genetic heritage of future generations. At first sight, the development of the induced reproduction technique involving the quality of the gametes may seem easy, elementary and simplistic, but it is not. Little is known about the reproduction of that "surubim", whose broodfishes coming from extremely impacted areas must be produced "urgently". Considering the facts mentioned above, it is our intention to study the characteristics of the semen through subjective methods associated to more precise ones (computational methods), as well as its conservation during short stocking periods and at different temperatures. Furthermore, it is also essential to improve the techniques that make the use of oocytes and eggs viable, characterizing them morphologically, before and after hormonal induction, assess the reproductive potential (fertilization, hatching and larvae), as well as the biotechnology of cryopreservation of these gametes and future embryos, parameters that are usually employed in commercial fish farms, and which will be validated here by science. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OKAWARA, RENAN YOSHIHARU; SANCHES, EDUARDO ANTONIO; CANEPPELE, DANILO; DAMASCENO, DANIELLE ZANERATO; ROMAGOSA, ELIZABETH. Ovulation and initial rearing of Steindachneridion parahybae (Siluriformes: Pimelodidae) larvae from different accumulated thermal units. ICHTHYOLOGICAL RESEARCH, v. 62, n. 4, p. 495-503, NOV 2015. (11/02818-5, 11/04780-5)

LOPES, TAIS DA SILVA; SANCHES, EDUARDO ANTONIO; OKAWARA, RENAN YOSHIHARU; ROMAGOSA, ELIZABETH. Chilling of Steindachneridion parahybae (siluriformes: pimelodidae) embryos. Theriogenology, v. 84, n. 4, p. 538-544, SEP 1 2015. (11/02818-5)

SANCHES, E. A.; MARCOS, R. M.; OKAWARA, R. Y.; CANEPPELE, D.; BOMBARDELLI, R. A.; ROMAGOSA, E.. Sperm motility parameters for Steindachneridion parahybae based on open-source software. Journal of Applied Ichthyology, v. 29, n. 5, p. 1114-1122, OCT 2013. (11/02818-5, 09/18609-6)

SANCHES, E. A.; OKAWARA, R. Y.; CANEPPELE, D.; TOLEDO, C. P. R.; BOMBARDELLI, R. A.; ROMAGOSA, E.. Sperm characteristics of Steindachneridion parahybae (Steindachner, 1877) throughout 112h of storage at four temperatures. Journal of Applied Ichthyology, v. 31, p. 10-pg., 2015-06-01. (09/18609-6, 11/02818-5)

SANCHES, E. A.; OKAWARA, R. Y.; CANEPPELE, D.; TOLEDO, C. P. R.; BOMBARDELLI, R. A.; ROMAGOSA, E.. Sperm characteristics of Steindachneridion parahybae (Steindachner, 1877) throughout 112h of storage at four temperatures. Journal of Applied Ichthyology, v. 31, n. 1, SI, p. 79-88, JUN 2015. (11/02818-5, 09/18609-6)