Detection of mollicutes in the female urogenital tract and its relation to genetic polymorphisms and expression of cytokines in women of Vitoria da Conquista, BA

• Grant number: 11/10138-4
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2011
• End date: January 31, 2014
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Jorge Timenetsky
• Grantee: Jorge Timenetsky
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Lucas Miranda Marques

Abstract

Many mollicutes are seen as constituting the healthy individual normal microflora. However, some were also associated with urogenital infections, complicating the understanding of the role of these species. Some factors are considered when assessing its pathogenic potential. In female urogenital tract, species of Mycoplasma and Ureaplasma are also commonly implicated as causing infections, changes perigestacionais and reproduction disorders such as infertility, miscarriage, stillbirth, premature births and underweight children. Some studies have reported the cytokine polymorphisms with relation to the colonization of microorganisms in the female genital tract. Based on these data, the aims of this study were detect mollicutes and relate to genetic polymorphism and cytokine expression in sexually active women with or without genital disorders in Vitória da Conquista, Bahia. The total sample of this study will be composed of 300 women, being 200 with genital disorder and 100 without symptoms. Cervical swab and peripheral blood samples will be collected. The cervical samples will be used for detection of Mycoplasma hominis, M. penetrans, M. genitalium, U. urealyticum and U. parvum using PCR and Real Time PCR. Blood samples will be used for polymorphisms and cytokine evaluation. There is no known incidence of these bacteria in the urogenital tract of the population being studied, as well, the genetic polymorphism of cytokines in patients infected with mycoplasmas and ureaplasmas. This study is expected to provide data for a preventive action in the spread of these microorganisms, promoting the reduction of costs with genital infections. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAMPOS, GUILHERME BARRETO; LOBAO, TASSIA NEVES; SELIS, NATHAN NEVES; AMORIM, ALINE TEIXEIRA; MARTINS, HELLEN BRAGA; BARBOSA, MAYSA SANTOS; CALDEIRA OLIVEIRA, THIAGO HENRIQUE; DOS SANTOS, DJANILSON BARBOSA; FIGUEIREDO, TIANA BAQUEIRO; MARQUES, LUCAS MIRANDA; et al. Prevalence of Mycoplasma genitalium and Mycoplasma hominis in urogenital tract of Brazilian women. BMC INFECTIOUS DISEASES, v. 15, FEB 14 2015. (11/10138-4)

LOBAO, T. N.; CAMPOS, G. B.; SELIS, N. N.; AMORIM, A. T.; SOUZA, S. G.; MAFRA, S. S.; PEREIRA, L. S.; DOS SANTOS, D. B.; FIGUEIREDO, T. B.; MARQUES, L. M.; et al. Ureaplasma urealyticum and U. parvum in sexually active women attending public health clinics in Brazil. EPIDEMIOLOGY AND INFECTION, v. 145, n. 11, p. 2341-2351, AUG 2017. (11/10138-4)