Identifying biomarkers for embryo biotechnologies through proteomics and lipid fingerprinting of bovine embryos

• Grant number: 10/01077-9
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: September 01, 2011
• End date: November 30, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Roseli Fernandes Gonçalves
• Grantee: Roseli Fernandes Gonçalves
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant(s): 12/08569-0 - Identifying biomarkers for embryo biotechnologies through proteomics and lipid fingerprinting of bovine embryos, AV.EXT
• Associated scholarship(s): 14/04241-5 - Lipid profile of female and male embryos produced in vitro, BP.IC; 11/18085-7 - Identifying biomarkers for embryo biotechnologies through proteomics and lipid fingerprinting of bovine embryos, BP.JP

Abstract

Although currently the national livestock has reached a high technological level, certain factors affect the reproductive performance of animals. Thus, lowering productivity, profitability, and therefore the competitiveness of livestock. The biggest limitation to reproductive efficiency among mammals is embryonic mortality, estimated at 25-60%. The quality and survival of embryos produced in vitro, as well as by nuclear transfer of somatic cells are lower than those of embryos generated in vivo. Little is known of the changes in gene expression and protein and lipid profile found in pre-implantation embryos obtained from bovine in vitro fertilization and nuclear transfer of somatic cells when compared with those obtained by embryo transfer. This project proposes to investigate the role of proteins and lipids in embryo mortality and the effect of different embryo biotechnologies about this process, aimed at: 1) characterize differences in protein profile of cattle Bos taurus and Bos indicus pre-implantation embryos derived from in vitro fertilization, embryo transfer and nuclear transfer of somatic cells and systematize the information obtained in order to choose candidate proteins to carry out deeper analysis; 2) identify the proteins of interest; 3) identify possible variations in protein and lipid profile related to the sex of embryos obtained by nuclear transfer of somatic cells; 4) characterize the fatty acid composition of pre-implantation embryos; 5) identify biomarkers associated with embryonic loss. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GUERREIRO, TATIANE MELINA; GONCALVES, ROSELI FERNANDES; RODRIGUES MELO, CARLOS FERNANDO O.; DE OLIVEIRA, DIOGO NOIN; LIMA, ESTELA DE OLIVEIRA; VISINTIN, JOSE ANTONIO; DE ACHILLES, MARCOS ANTONIO; CATHARINO, RODRIGO RAMOS. A Metabolomic Overview of Follicular Fluid in Cows. FRONTIERS IN VETERINARY SCIENCE, v. 5, FEB 8 2018. (15/06809-1, 11/18085-7, 11/50400-0, 16/17066-2, 10/01077-9)

GONCALVES, ROSELI F.; FERREIRA, MONICA S.; DE OLIVEIRA, DIOGO N.; CANEVAROLO, RAFAEL; ACHILLES, MARCOS A.; D'ERCOLE, DANIELA L.; BOLS, PETER E.; VISINTIN, JOSE A.; KILLIAN, GARY J.; CATHARINO, RODRIGO R.. Analysis and characterisation of bovine oocyte and embryo biomarkers by matrix-assisted desorption ionisation mass spectrometry imaging. REPRODUCTION FERTILITY AND DEVELOPMENT, v. 28, n. 3, p. 293-301, 2016. (10/01077-9, 11/18085-7)