Clinical and laboratory investigation of the antioxidant effects of autologous plasma used in equine joint diseases

• Grant number: 11/12929-9
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2011
• End date: October 31, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Raquel Yvonne Arantes Baccarin
• Grantee: Raquel Yvonne Arantes Baccarin
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Cristina de Oliveira Massoco Salles Gomes ; Luis Cláudio Lopes Correia da Silva ; Thais Sodre de Lima Machado ; Yara Maria Corrêa da Silva Michelacci

Abstract

Joint disease as result of imbalances in the relationship between oxidants and antioxidants have been little studied in horses, unlike that found in the abundant literature focused on the human species, where several studies have demonstrated the role of reactive oxygen species (ROS) in inflammatory articular states, chondrocyte senescence, the articular cartilage extracellular matrix degradation and decreasing of synovial fluid viscosity. This study aims to investigate the composition of antioxidant agents and mechanisms present in the normal horses synovial fluid, horses with osteoarthritis (OA) or osteochondrosis (OC), and the involvement of oxidative stress in these diseases. Still, it will be check (or not) relationship between oxidative stress and inflammation in OA and OC. In the final step, we intend to test "in vivo" effects of the administration of autologous plasma in equine joints affected by these diseases. For that, the synovial fluid of 30 horses will be collected in two phases of the experiment. Initially, six horses will receive autologous plasma in the metacarpophalangeal or tibio-tarsal healthy joint and the same amount of saline in the contralateral joint. The synovial fluid will be collected prior to application (time 0) and after 3, 6, 24 and 48 hours to determine the concentrations of prostaglandin E2 (PGE2), interleukin-1 (IL-1), antagonist of interleukin-1 receptor (IL-ra), tumor necrosis factor-2 (TNF2), hyaluronic acid and chondroitin sulfate. In a second phase, the synovial fluid of 12 horses (six with OA and six others with OC) will be collected prior to and 48 hours after arthroscopy. Similarly, the synovial fluid of another 12 horses (six with OA and six others with OC) will be collected prior to arthroscopy and 48 hours, however these animals will receive autologous plasma at the end of the arthroscopic procedure. Will be held the following determinations in these synovial fluids: activity of superoxide dismutase (SOD), glutathione peroxidase activity (GSH-Px), catalase activity (CAT), total antioxidant capacity (TAC), levels of ascorbic acid, isoprostane concentration, concentration of carbonyl groups, prostaglandin E2, concentration of hyaluronic acid and chondroitin sulfate. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACHADO, THAIS S. L.; MASSOCO, CRISTINA O.; SILVA, LUIS CLAUDIO L. C.; FULBER, JOICE; MOREIRA, JULIANA J.; BACCARIN, RAQUEL Y. A.. Effects of blood-derived products and sodium hyaluronate on equine synovial fluid cells and on synovial fluid from osteochondrotic joints of horses after arthroscopy and administration of treatment. AMERICAN JOURNAL OF VETERINARY RESEARCH, v. 80, n. 7, p. 646-656, JUL 2019. (11/19096-2, 11/12929-9)