Effect of low level laser on C2C12 muscle cells subjected to injury by Bothrops jararacussu venom

• Grant number: 11/14376-7
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2011
• End date: November 30, 2013
• Field of knowledge: Biological Sciences - Pharmacology - General Pharmacology
• Principal Investigator: Stella Regina Zamuner
• Grantee: Stella Regina Zamuner
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
• Associated researchers: José Carlos Cogo ; Raquel Agnelli Mesquita Ferrari

Abstract

The venom of Bothrops species induces a local inflammatory reaction characterized by pain, edema, leukocyte migration and necrosis. The antivenom is used to neutralize the greatest possible amount of circulating venom, thus minimizing its systemic effects, but its action does not extend the local manifestations, therefore, it is necessary the use of another therapeutic approach to control this event. The low-power laser therapy is an alternative treatment in cases of muscle damage due to their biological effects, such as analgesics, anti-inflammatory and healing and is considered a bio-stimulant use in tissue.This project will study the effect of low power laser in muscle cells subjected to injury by Bothrops jararacussu venom. Thus, tests are carried out for verifying the integrity of the monolayer cell viability, differentiation and protein expression of MHC on C2C12 muscle cells. Thus, this study will better understand the pathophysiological effects of bothropic envenoming as well as contribute to a better understanding of the effects of low-power laser, and eventually, promote a therapeutic approach more efficient and/or complementary. (AU)