Biochemical profile, including acute phase proteins and immunoglobulins, microbial condition and somatic cell count in Jaffarabadi primiparous and pluriparous buffaloes over complete lactation

• Grant number: 11/19480-7
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2012
• End date: January 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: José Jurandir Fagliari
• Grantee: José Jurandir Fagliari
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The aim of the study is to evaluate the biochemical profile, including acute phase proteins and immunoglobulins, the population of microorganisms and somatic cell counts in colostrum / milk from 30 Jaffarabadi buffaloes, primiparous and pluriparous, which will be followed monthly during complete lactation. Before taking the samples of milk, physical examination of the mammary gland, strip cup test and California Mastitis Test (CMT) for the diagnosis of inapparent mastitis (subclinical) will be conducted. After asepsis of the udder, 50 mL of milk samples from each quarter will be collected into sterile plastic bottles without preservative, for the microbiological isolation, biochemical profile and proteic fractionation in polyacrylamide gel containing sodium dodecyl sulfate (SDS -PAGE) and 50 mL of milk samples from each quarter will be collected into sterile plastic bottles containing a appropriate preservative for somatic cell count (SCC), an electronic counter infrared. In addition, the antibiotic sensitivity profile of microorganisms isolated from milk samples will assess. The data will be submitted to descriptive statistical analysis and the values and expressed as mean and standard deviation will be analyzed by the Tukey's test, after checking the homogeneity of the samples (Zar, 1999). The significance will be checked at 5% probability. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LUCAS J.L. PIZAURO; DANIELA G. DA SILVA; ANDRÉ M. SANTANA; STELLA M.A. MORAIS; NATÁLIA C. BENINCASA; JOSÉ J. FAGLIARI. Perfil bioquímico, inclusive proteinograma, do soro lácteo de búfalas primíparas e pluríparas sadias ao longo da lactação. Pesquisa Veterinária Brasileira, v. 34, p. 79-86, 2014-12-00. (11/19480-7, 11/03514-0)

PIZAURO, L. J. L.; SILVA, D. G.; SANTANA, A. M.; CLEMENTE, V.; LARA, G. H. B.; LISTONI, F. J. P.; VAZ, A. C. N.; VIDAL-MARTINS, A. M. C.; RIBEIRO, M. G.; FAGLIARI, J. J.. Prevalência e etiologia da mastite bubalina e contagem de células somáticas no leite, nas estações seca e chuvosa, em um rebanho bubalino do município de Analândia, Estado de São Paulo, Brasil. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 66, n. 6, p. 1703-1710, DEC 2014. (11/19480-7, 11/03514-0)