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Milk microbiological and biochemical study and blood biochemical profile, including acute phase proteins and immunoglobulins, in goats mastitis

Grant number: 13/05507-6
Support type:Regular Research Grants
Duration: June 01, 2013 - May 31, 2015
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal Investigator:José Jurandir Fagliari
Grantee:José Jurandir Fagliari
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Milk from goats has been increasingly used as medicinal food and it engenders high valued byproducts, as cheese, which has a good market price. A limiting factor to the dairy industry is the presence of diseases, especially mastitis, which when not treated can turn into a serious problem with considerable economic losses. Researches along the last years suggest that in the future quantification of highly sensitive inflammation markers, acute-phase proteins (APPs), will be used in a daily basis in the veterinary routine to evaluate an animal´s or herd state of health, optimize productive performance, monitor the efficacy of antibiotic therapies and detect silent diseases, such as subclinical mastitis. In spite of the value of APPs quantifications in the clinical routine of goats, there are few information disposable in the scientific literature. Therefore the aim of this study was to evaluate the changes in milk using the strip cup test, California Mastitis Test, somatic cell count (SCC) and microbiological profile and also determine the biochemical profile, especially APPs, in milk and blood serum goats with naturally acquired or subclinical mastitis. Before milking the mammary glands will be evaluated through inspection and palpation. Then the strip cup test and California Mastitis Test will be performed. Subsequently asepsis of the teat and sampling of milk will be performed for later SCC, microbiological culture and biochemical profile. Blood samples will be collected through jugular vein puncture, in plain tubes. After clot retraction the sample will be centrifuged and the aliquots will be stored in a -20ºC freezer for later analysis. (AU)