| Grant number: | 10/20186-3 |
| Support Opportunities: | Regular Research Grants |
| Start date: | April 01, 2012 |
| End date: | February 28, 2015 |
| Field of knowledge: | Health Sciences - Dentistry - Endodontics |
| Principal Investigator: | Flaviana Bombarda de Andrade |
| Grantee: | Flaviana Bombarda de Andrade |
| Host Institution: | Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil |
| City of the host institution: | Bauru |
| Associated researchers: | Marco Antonio Hungaro Duarte |
Abstract
The endodontic microbiota is mainly anaerobic and proteolitic, due to this site conditions and disposable nutrients. Factors of adherence can promote the microbial installation at the root canal walls, forming biofilms, and also proliferation inside the dentinal tubules. The biofilm microorganisms change genetic material, increasing their resistance. Because of this, it is almost impossible a microbial clinical isolate having the genes totally equal to the pattern strains of species of culture collections, like ATCC (American Type Culture Collection). The aim of this study is investigate three species of common microorganisms of necrotic root canals, comparing five clinical isolates to the respective ATCC strain, evaluating some characteristics of planctonic and biofilm strains. The investigated anaerobic species will be Fusobacterium nucleatum and Bifidobacterium dentium, besides the facultative Enterococcus faecalis. The clinical isolates will be cultivated in culture media, isolated and identified by biochemical commercial tests (System Api, Bio-Meriéux, França). The static biofilms will be produced in celluloses' membrane, characterized by scanning electron microscopy (SEM), measured their volume by confocal microscopy and by counting of colonies at the microbial culture (colony forming units - CFU). Each species has specifics genes of adherence, which is an advantageous factor. The expression of these specific genes will be evaluated by technique of Real-time PCR, its major or minor production by planctonic bacteria or biofilm, from ATCC strain or clinical isolate. The accuracy and knowledge of the root canals microorganisms' behavior can promote better study models of antimicrobial effect of substances in the future, besides better knowledge of virulence of microorganisms. (AU)
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