Utilization of molecular markers sequences for identifying tree species in the São Paulo State

• Grant number: 11/22923-8
• Support Opportunities: Regular Research Grants
• Field of knowledge: Biological Sciences - Botany - Plant Taxonomy
• Principal Investigator: Vinicius Castro Souza
• Grantee: Vinicius Castro Souza
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated researchers: Ricardo Ribeiro Rodrigues

Abstract

The present work aims to build a database of rbcL sequences for 500 tree species from São Paulo state and test the efficiency using this molecular marker in plant identification. In the specific case of Lauraceae, Myrtaceae and Fabaceae families, will be included also the matK and trnH-psbA markers. This database will intend to be a tool for plant identification when the biological material is insufficient to confirm the naming. Therefore, 500 São Paulo state arboreal species will be collected through the leaves and obtained rbcL sequences. After mounting the database, will be incorporated sequences from other specimens and tests the performed to verify the effectiveness this method in tree species identification, with Parsimony and Neighbour-joining analysis and checking the percentage of correct identification (PCI) in the specific, generic, and family levels. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA DE LIMA, RENATO A.; DE OLIVEIRA, ALEXANDRE ADALARDO; DALLA COLLETTA, GABRIEL; FLORES, THIAGO BEVILACQUA; GAYOSO COELHO, RUBENS L.; DIAS, PEDRO; FREY, GABRIEL PONZONI; IRIBAR, AMAIA; RODRIGUES, RICARDO RIBEIRO; SOUZA, VINICIUS CASTRO; et al. Can plant DNA barcoding be implemented in species-rich tropical regions? A perspective from Sao Paulo State, Brazil. GENETICS AND MOLECULAR BIOLOGY, v. 41, n. 3, p. 661-670, JUL-SEP 2018. (11/22923-8, 15/00682-0, 99/09635-0)