The effect of intact and whey protein hydrolysate consumption on glucose transporters 1 and 4 in rats

• Grant number: 12/05859-7
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2012
• End date: June 30, 2014
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Jaime Amaya Farfan
• Grantee: Jaime Amaya Farfan
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

This proposal will attempt to verify the hypothesis that both the whole dietary milk whey protein and its enzymatic hydrolyzate have different abilities to induce the activation of the Na-independent muscle glucose transporter GLUT4 in the rat. The hypothesis is based on a series of previous observations of our research group, as well as others from abroad, and its confirmation may explain the biochemical and physiological differences that have been shown to exist between these two forms of the protein, when fed to the rat. Additionally, this is a case in which the technological modification of a dietary protein could bring an advantage to patients of diabetes type 1. The Westernblot technique shall be used to determine the levels of GLUT1 and GLUT4, while common assays will serve to monitor the classical serum and muscle biochemical markers. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORATO, PRISCILA NEDER; BARBOZA LOLLO, PABLO CHRISTIANO; MOURA, CAROLINA SOARES; BATISTA, THIAGO MARTINS; CAMARGO, RAFAEL LUDEMANN; CARNEIRO, EVERARDO MAGALHAES; AMAYA-FARFAN, JAIME. Whey Protein Hydrolysate Increases Translocation of GLUT-4 to the Plasma Membrane Independent of Insulin in Wistar Rats. PLoS One, v. 8, n. 8, AUG 30 2013. (12/05859-7)

MORATO, P. N.; LOLLO, P. C. B.; MOURA, C. S.; BATISTA, T. M.; CARNEIRO, E. M.; AMAYA-FARFAN, J.. A dipeptide and an amino acid present in whey protein hydrolysate increase translocation of GLUT-4 to the plasma membrane in Wistar rats. Food Chemistry, v. 139, n. 1-4, p. 853-859, JUL 1 2013. (12/05859-7)

ROQUETTO, ALINE RISSETTI; SANTANA MONTEIRO, NAICE ELEIDIANE; MOURA, CAROLINA SOARES; TORETI, VIVIANE CRISTINA; DE PACE, FERNANDA; DOS SANTOS, ANDREY; PARK, YONG KUN; AMAYA-FARFAN, JAIME. Green propolis modulates gut microbiota, reduces endotoxemia and expression of TLR4 pathway in mice fed a high-fat diet. Food Research International, v. 76, p. 8-pg., 2015-10-01. (12/05859-7)

ROQUETTO, ALINE RISSETTI; SANTANA MONTEIRO, NAICE ELEIDIANE; MOURA, CAROLINA SOARES; TORETI, VIVIANE CRISTINA; DE PACE, FERNANDA; DOS SANTOS, ANDREY; PARK, YONG KUN; AMAYA-FARFAN, JAIME. Green propolis modulates gut microbiota, reduces endotoxemia and expression of TLR4 pathway in mice fed a high-fat diet. Food Research International, v. 76, n. 3, p. 796-803, OCT 2015. (12/05859-7)

MONTEIRO, NAICE E. S.; ROQUETTO, ALINE R.; DE PACE, FERNANDA; MOURA, CAROLINA S.; DOS SANTOS, ANDREY; YAMADA, AUREO T.; SAAD, MARIO JOSE A.; AMAYA-FARFAN, JAIME. Dietary whey proteins shield murine cecal microbiota from extensive disarray caused by a high-fat diet. Food Research International, v. 85, p. 121-130, JUL 2016. (13/02862-0, 12/05859-7)