Microwaves, biocatalysis, divalent metal ions and nanotechnology in peptide synthesis

• Grant number: 12/09068-4
• Support type: Regular Research Grants
• Duration: July 01, 2012 - December 31, 2014
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Maria Teresa Machini
• Grantee: Maria Teresa Machini
• Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Our proposal is to continue the part of our research that focus synthetic chemistry, peptide synthesis, and was supported by FAPESP up to last November. Such research includes three ongoing studies and has the following goals: (i) speed-up and improvement of chemical solid-phase peptide synthesis, technology of choice small-scale production of such compound; (ii) establisment of alternative synthetic technologies that are cleaner, cheaper and/or more selective; (iii) increase of the knowledge about them. More specifically, we want to complement our previous data and investigations on: 1) positive and negative effects of conventional heating and microwaves on different steps of solid-phase peptide synthesis; 2) imobilization of proteases on superparamagnetic nanoparticles with the aim to improve their properties and use the immobilized enzymes as catalysts in peptide synthesis; 3) detachment of the peptide from resin through solvolysis mediated by divalent metal ions, procedure developed in our laboratory that can be used for the synthesis of C-terminal modified peptides. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROMANO, RENATO LAHOS; LIRIA, CLEBER WANDERLEI; TERSA MACHINI, M.; COLEPICOLO, PIO; ZAMBOTTI-VILLELA, LEONARDO. Cadmium decreases the levels of glutathione and enhances the phytochelatin concentration in the marine dinoflagellate Lingulodinium polyedrum. JOURNAL OF APPLIED PHYCOLOGY, v. 29, n. 2, p. 811-820, APR 2017. Web of Science Citations: 6.

ALTA, ROXANA Y. P.; VITORINO, HECTOR A.; GOSWAMI, DIBAKAR; LIRIA, CLEBER W.; WISNOVSKY, SIMON P.; KELLEY, SHANA O.; MACHINI, M. TERESA; ESPOSITO, BRENO P. Mitochondria-penetrating peptides conjugated to desferrioxamine as chelators for mitochondrial labile iron. PLoS One, v. 12, n. 2 FEB 8 2017. Web of Science Citations: 8.

CARVALHO, L. A. C.; REMUZGO, C.; PEREZ, K. R.; MACHINI, M. T. Hb40-61a: Novel analogues help expanding the knowledge on chemistry, properties and candidacidal action of this bovine alpha-hemoglobin-derived peptide. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v. 1848, n. 12, p. 3140-3149, DEC 2015. Web of Science Citations: 3.

UNGARO, VITOR A.; LIRIA, CLEBER W.; ROMAGNA, CAROLINA D.; COSTA, NATALIA J. S.; PHILIPPOT, KARINE; ROSSI, LIANE M.; TERESA MACHINI, M. A green route for the synthesis of a bitter-taste dipeptide combining biocatalysis, heterogeneous metal catalysis and magnetic nanoparticles. RSC ADVANCES, v. 5, n. 46, p. 36449-36455, 2015. Web of Science Citations: 4.

LIRIA, CLEBER W.; UNGARO, VITOR A.; FERNANDES, RAPHAELLA M.; COSTA, NATALIA J. S.; MARANA, SANDRO R.; ROSSI, LIANE M.; TERESA MACHINI, M. Synthesis, properties, and application in peptide chemistry of a magnetically separable and reusable biocatalyst. JOURNAL OF NANOPARTICLE RESEARCH, v. 16, n. 11 NOV 2014. Web of Science Citations: 4.