Development of optic system associate to the research of molecular markers for cancer diagnosis o in real time and minimum invasive

• Grant number: 09/16782-2
• Support type: Research Grants - Visiting Researcher Grant - International
• Duration: June 01, 2010 - May 31, 2011
• Field of knowledge: Engineering - Biomedical Engineering
• Principal researcher: Airton Abrahao Martin
• Grantee: Airton Abrahao Martin
• Visiting researcher: Karl J. Jalkanen
• Visiting researcher institution: Technical University of Denmark (DTU), Denmark
• Home Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil

Abstract

The main goal of this research is to use our prior knowledge obtained in our in vitro studies of cancer by the FT-Raman spectroscopy technique in order to enable the development of an optical system associated with the search for molecular markers for in vivo diagnosis of cancer in time real and minimally invasive. This study will be conducted in a high incidence of tumors and / or mortality rate in Brazil: colo-rectal, breast tumors, including melanoma skin tumors, polyps and, as well, as tumors of the thyroid. Since recent advances in biophotonics techniques (optical spectroscopy) and molecular biology have allowed the use of techniques capable of high resolution for the differentiation between healthy tissues and modified, as well as, for the discovery of molecular markers, this project will use in conjunction, the techniques of Raman spectroscopy and PCR. To achieve this goal, we will develop a compact dispersive Raman system coupled to a laser excitation at 785nm and a CCD detector for in vivo analysis of the suspect tissue. The groups of volunteers in this study will come from the collaborating institutions (INCA, UNICAMP, UNIFESP and AC Camargo Hospital). Spectral data obtained will be validated by conventional histological examination. With the use of a system Real-Time PCR studies as tumor markers, which will be useful in detecting cancer at an early stage in tumor assessment, monitoring of disease progression and determining the response to therapy. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PIVA, J. A. A. C.; SILVA, J. L. R.; RANIERO, L.; MARTIN, A. A.; BOHR, H. G.; JALKANEN, K. J. Overview of the use of theory to understand infrared and Raman spectra and images of biomolecules: colorectal cancer as an example. THEORETICAL CHEMISTRY ACCOUNTS, v. 130, n. 4-6, p. 1261-1273, DEC 2011. Web of Science Citations: 11.