Protein and lipid profile by mass spectrometry of infected cattle embryos with Bovine Herpesvirus 1 (BoHV-1)

• Grant number: 10/18476-3
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2011
• End date: May 31, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Magali D'angelo
• Grantee: Magali D'angelo
• Host Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
• Associated researchers: José Antonio Visintin ; Marcos Nogueira Eberlin ; Roseli Fernandes Gonçalves

Abstract

Although currently the national livestock has reached a high technological level, certain factors affect the reproductive performance of animals. Thus, lowering productivity, profitability, and therefore the competitiveness of livestock. The biggest limitation to reproductive efficiency among mammals is embryonic mortality, estimated at 25-60%, and abortion related to animal health. Little is known of the changes in gene expression and protein and lipid profile found in cattle pre-implantation embryos infected with Bovine Herpesvirus 1 (BoHV-1). This project proposes to investigate the role of proteins and lipids in embryo mortality associated with BoHV-1, aiming to: 1) characterize differences in protein profile of global cattle pre-implantation embryos infected or not with BoHV-1 and systematize the information obtained in order to choose candidate proteins to carry out further analysis; 2) identify proteins of interest; 3) characterize the fatty acid composition of pre-implantation embryos infected or not with BoHV-1; 4) identify biomarkers associated with embryonic loss and to indicate how likely the evolution of BoHV-1. (AU)