Influence of chronic alcoholism and estrogen deficiency on bone metabolism in the femur of rats

• Grant number: 12/50023-4
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2012
• End date: June 30, 2015
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Rosilene Fernandes da Rocha
• Grantee: Rosilene Fernandes da Rocha
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Excessive consumption of alcohol is an important public health problem and also presents a direct relationship with bone loss by decreasing bone turnover, decreased rates of bone resorption and osteogenesis. The estrogen deficiency is directly associated with osteoporosis, since it leads to increased osteoclast formation and decreased synthesis of osteoblasts, causing an imbalance in bone remodeling. The combination of osteoporosis and consumption of alcohol may have synergistic, deleterious effect on bone tissue and has been the subject of previous studies. The objective of this project is to investigate possible changes in bone metabolism in the femurs of rats subjected to chronic alcoholism and estrogen deficiency induced by ovariectomy. Ninety femurs already embedded in paraffin will be used, from rats treated in six different groups, equally divided according to the type of diet and the presence or absence of hormones. Group 1: simulated ovariectomy (Sham), and water-free diet, Group 2: Sham and daily doses of 20% of the calories in alcohol, Group 3: Sham and isocaloric feeding, provided through an aqueous solution of sucrose, Group 4: ovariectomy (Ovx), free water and solid diet, Group 5: Ovx and 20% of calories in alcohol, Group 6: Ovx and isocaloric diet. The femurs are evaluated according to quantitative reading of the slides for immunohistochemical analysis, using markers of RANKL (N-19: sc-7628), Bone sialoprotein (BSP - LF - 87), osteocalcin (OCC - FL -110 : sc-30044) and Anti PARP p-85. The values obtained will be tested using ANOVA and Tukey's test with a significance level of 5%. (AU)