Design of integrative and separative strategies for the primary recovery of industrial bioproducts

• Grant number: 12/50921-2
• Support Opportunities: Regular Research Grants
• Start date: January 01, 2013
• End date: December 31, 2015
• Field of knowledge: Engineering - Chemical Engineering - Chemical Process Industries
• Agreement: CONICET
• Principal Investigator: Adalberto Pessoa Junior
• Grantee: Adalberto Pessoa Junior
• Principal researcher abroad: Bibiana Beatriz Nerli
• Institution abroad: Universidad Nacional de Rosario (UNR), Argentina
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 12/12022-6 - Extraction of monoclonal antibody fragments type scFv by aqueous two-phase micellar systems, AP.R

Abstract

Our researcher group has a project in progress, approved by FAPESP (2012/12022-6), directed to the evaluation of micellar aqueous two-phase systems extraction of a recombinant antibody scFv anti- LDL (main identified atherosclerosis autoantigen) produced by the genetically modified yeast Pichia Pastoris. These systems show several advantages like fast phases separation, easy scale-up and low cost. The cooperation with the group of Dra. Nerli, with experience in protein purification from different biological resources by integrative methodologies as aqueous two-phase systems and affinity mechanisms, will be of great importance to the incorporation of novel biomolecules separation strategies in addition to the formation of human resources in the bioseparative thematics. (AU)