Advanced search
Start date
Betweenand

Extraction of monoclonal antibody fragments type scFv by aqueous two-phase micellar systems

Grant number: 12/12022-6
Support type:Regular Research Grants
Duration: October 01, 2012 - September 30, 2014
Field of knowledge:Engineering - Chemical Engineering
Principal Investigator:Adalberto Pessoa Junior
Grantee:Adalberto Pessoa Junior
Home Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers:Dulcineia Saes Parra Abdalla ; Maria Elena Santos Taqueda ; Pedro de Alcântara Pessôa Filho
Associated grant(s):12/50921-2 - Design of integrative and separative strategies for the primary recovery of industrial bioproducts, AP.R

Abstract

Owing to a high percentage (70-80% correspond to the separation and purification steps) of the costs to obtain biotechnological products, it is necessary to optimize some methodologies to ensure a good cost/benefit relationship. Nowadays, the micellar aqueous two-phases systems are considered as an appropriated bio-separating technology because their properties are suitable to maintain the macromolecule's integrity and also allow regulating the experimental conditions enabling a selective extraction. In this project, the liquid-liquid extraction by micellar aqueous two-phase systems, composed by Triton X-114/ buffer McIlvaine, will be studied as a method to separate and to purify scFv antibodies from Pichia pastoris cultures. The effect of the system's components on the antibody integrity will be studied as well as the effect of different variables as pH, temperature, surfactant concentration, salts concentration and the presence of ligand molecules over the partition and purification of the scFv antibody. Finally, the best experimental conditions will be selective to apply a 1000-times scaling in order to test their potential for industrial application. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VICENTE, FILIPA A.; MALPIEDI, LUCIANA P.; E SILVA, FRANCISCA A.; PESSOA, JR., ADALBERTO; COUTINHO, JOAO A. P.; VENTURA, SONIA P. M. Design of novel aqueous micellar two-phase systems using ionic liquids as co-surfactants for the selective extraction of (bio)molecules. Separation and Purification Technology, v. 135, p. 259-267, OCT 15 2014. Web of Science Citations: 35.
DUTRA-MOLINO, J. V.; FEITOSA, V. ARAUJO; DE LENCASTRE-NOVAES, L. C.; SANTOS-EBINUMA, V. DE CARVALHO; LOPES, A. MORENI; JOZALA, A. FAUSTINO; VIANA MARQUES, D. DE ARAUJO; MALPIEDI, L. PELLEGRINI; PESSOA JUNIOR, A. BIOMOLECULES EXTRACTED BY ATPS: PRACTICAL EXAMPLES. REVISTA MEXICANA DE INGENIERIA QUIMICA, v. 13, n. 2, p. 359-377, AUG 2014. Web of Science Citations: 9.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.