Development of products and processes with supercritical technology: construction of one multipurpose unit to obtain extracts of industrial interest.

• Grant number: 12/51317-1
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2013
• End date: February 28, 2015
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Engineering
• Principal Investigator: Fernando Antonio Cabral
• Grantee: Fernando Antonio Cabral
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

To extract and fractionate phenolic compounds from matrix of plant origin, extracts of leaves of Baccharis dracunculifolia will be obtained by supercritical extraction in fixed bed with supercritical carbon dioxide (scCO2) at 60ºC and 400 bar and after they will be fractionated in separators, by pressure reducing, stage by stage from 300 bar to 80 bar, hydroalcoholic extracts of green propolis, of red propolis, of Eugenia uniflora leaves and of crajurú leaves (Arrabidaea chica) will be obtained by conventional methods and will after be fractionated selectively into separators by gradual reduction of pressure by using supercritical carbon dioxide as antisolvent. Aiming also extract and fractionate saponins, extracts of P. glomerata roots, the roots of Pfaffia paniculata and fruits of Melia azedarach, rich in saponins, will initially be produced by Soxhlet extraction with polar solvents and then will be fractionated using supercritical technology in two ways : 1) the fractionation of the dried extract using a fixed bed sequential extraction with supercritical carbon dioxide (scCO2), scCO2/etanol and ethanol, and 2) the fractionation by precipitation from a water-alcohol solution using scCO2 as anti-solvent, a sequence of separators at 50ºC where the pressure is reduced from stage to stage 300 bar to 80 bar. In the latter case, the hydroalcoholic solution is produced from the resolubilization of dry extracts of Pfaffia and a solution of Melia ethanol: water 70:30 v:v. Ali samples will be characterized as income from extraction and fractionation and presence of phenolics, flavonoids, artepillin C (Bacharis and propolis) and antioxidant activity, anthocyanins in Arrabidaea, saponins in the roots of P. glomerata and P. paniculata and fruits of Melia azedarach. To this end, we intend to build a multi-purpose unit to be able to study different cases of extraction (conventional or scCO2) and conventional fractionation or supercritical extracts using supercritical technology, development and process optimization and scale (scale up) and able to produce extracts of industrial interest. (AU)

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BARROS, HELENA D. F. Q.; GRIMALDI, RENATO; CABRAL, FERNANDO A.. Lycopene-rich avocado oil obtained by simultaneous supercritical extraction from avocado pulp and tomato pomace. JOURNAL OF SUPERCRITICAL FLUIDS, v. 120, n. 1, p. 1-6, FEB 2017. (12/51317-1)

PAULA, JULIA T.; SOUSA, ILZA M. O.; FOGLIO, MARY A.; CABRAL, FERNANDO A.. Selective fractionation of extracts of Arrabidaea chica Verlot using supercritical carbon dioxide as antisolvent. JOURNAL OF SUPERCRITICAL FLUIDS, v. 133, n. 1, p. 9-16, MAR 2018. (15/08338-6, 12/51317-1)

PAULA, JULIA T.; AGUIAR, ANA C.; SOUSA, ILZA M. O.; MAGALHAES, PEDRO M.; FOGLIO, MARY A.; CABRAL, FERNANDO A.. Scale-up study of supercritical fluid extraction process for Baccharis dracunculifolia. JOURNAL OF SUPERCRITICAL FLUIDS, v. 107, p. 219-225, JAN 2016. (12/51317-1)

BARROS, HELENA D. F. Q.; COUTINHO, JANCLEI P.; GRIMALDI, RENATO; GODOY, HELENA T.; CABRAL, FERNANDO A.. Simultaneous extraction of edible oil from avocado and capsanthin from red bell pepper using supercritical carbon dioxide as solvent. JOURNAL OF SUPERCRITICAL FLUIDS, v. 107, p. 315-320, JAN 2016. (12/51317-1)

PAULA, JULIA T.; SOUSA, ILZA M. O.; FOGLIO, MARY A.; CABRAL, FERNANDO A.. Selective fractionation of supercritical extracts from leaves of Baccharis dracunculifolia. JOURNAL OF SUPERCRITICAL FLUIDS, v. 127, p. 62-70, SEP 2017. (12/51317-1)

MONROY, YANETH M.; RODRIGUES, RODNEY A. F.; SARTORATTO, ADILSON; CABRAL, FERNANDO A.. Purple corn (Zea mays L.) pericarp hydroalcoholic extracts obtained by conventional processes at atmospheric pressure and by processes at high pressure. Brazilian Journal of Chemical Engineering, v. 37, n. 1, p. 237-248, MAR 2020. (12/51317-1)

GARMUS, TABATA T.; KOPF, SARAH F. M.; PAULA, JULIA T.; AGUIAR, ANA C.; DUARTE, GUSTAVO H. B.; EBERLIN, MARCOS N.; CABRAL, FERNANDO A.. ETHANOLIC AND HYDROALCOHOLIC EXTRACTS OF PITANGA LEAVES (Eugenia uniflora L.) AND THEIR FRACTIONATION BY SUPERCRITICAL TECHNOLOGY. Brazilian Journal of Chemical Engineering, v. 36, n. 2, p. 1041-1051, APR-JUN 2019. (12/51317-1, 14/16662-5)

PAULA, JULIA T.; SOUSA, ILZA M. O.; FOGLIO, MARY A.; CABRAL, FERNANDO A.. Selective fractionation of extracts of Arrabidaea chica Verlot using supercritical carbon dioxide as antisolvent. JOURNAL OF SUPERCRITICAL FLUIDS, v. 133, p. 8-pg., 2018-03-01. (12/51317-1, 15/08338-6)

MONROY, YANETH M.; RODRIGUES, RODNEY A. F.; SARTORATTO, ADILSON; CABRAL, FERNANDO A.. Extraction of bioactive compounds from cob and pericarp of purple corn (Zea mays L.) by sequential extraction in fixed bed extractor using supercritical CO2, ethanol, and water as solvents. JOURNAL OF SUPERCRITICAL FLUIDS, v. 107, p. 250-259, JAN 2016. (12/51317-1)

PAULA, JULIA T.; SOUSA, ILZA M. O.; FOGLIO, MARY A.; CABRAL, FERNANDO A.. Solubility of protocatechuic acid, sinapic acid and chrysin in supercritical carbon dioxide. JOURNAL OF SUPERCRITICAL FLUIDS, v. 112, p. 89-94, JUN 2016. (12/51317-1)

MONROY, YANETH M.; RODRIGUES, RODNEY A. F.; SARTORATTO, ADILSON; CABRAL, FERNANDO A.. Influence of ethanol, water, and their mixtures as co-solvents of the supercritical carbon dioxide in the extraction of phenolics from purple corn cob (Zea mays L.). JOURNAL OF SUPERCRITICAL FLUIDS, v. 118, p. 11-18, DEC 2016. (12/51317-1)

MONROY, YANETH M.; RODRIGUES, RODNEY A. F.; SARTORATTO, ADILSON; CABRAL, FERNANDO A.. Optimization of the extraction of phenolic compounds from purple corn cob (Zea mays L.) by sequential extraction using supercritical carbon dioxide, ethanol and water as solvents. JOURNAL OF SUPERCRITICAL FLUIDS, v. 116, p. 10-19, OCT 2016. (12/51317-1)

BARROS, HELENA D. F. Q.; GRIMALDI, RENATO; CABRAL, FERNANDO A.. Lycopene-rich avocado oil obtained by simultaneous supercritical extraction from avocado pulp and tomato pomace. JOURNAL OF SUPERCRITICAL FLUIDS, v. 120, p. 6-pg., 2017-02-01. (12/51317-1)

MONROY, YANETH M.; RODRIGUES, RODNEY A. F.; RODRIGUES, MARILI V. N.; CABRAL, FERNANDO A.. Fractionation of ethanolic and hydroalcoholic extracts of green propolis using supercritical carbon dioxide as an anti-solvent to obtain artepillin rich-extract. JOURNAL OF SUPERCRITICAL FLUIDS, v. 138, p. 167-173, AUG 2018. (12/51317-1)