Effects of short-chain fatty acids produced by probiotic bacteria in the prophylaxis and treatment of allergic airway inflammation

• Grant number: 12/50410-8
• Support Opportunities: Research Grants - Young Investigators Grants
• Duration: June 01, 2013 - May 31, 2018
• Field of knowledge: Biological Sciences - Physiology
• Principal Investigator: Caroline Marcantonio Ferreira
• Grantee: Caroline Marcantonio Ferreira
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated grant(s): 21/06751-4 - Deciphering the immune mechanism of increased airway allergic disease in menopause, AP.JP2
• Associated scholarship(s): 14/03879-6 - Gut microbiota and experimental allergic asthma in C57BL/6 and A/J mice: genetic and fiber intake influence, BP.IC; 13/06265-6 - Role of acetate on experimental allergic asthma: short fatty acid chain productiong probiotic bacteria as a mechanism of action, BP.IC; 13/09473-9 - Effects of short-chain fatty acids produced by probiotic bacteria in the prophylaxis and treatment of allergic airway inflammation, BP.JP

Abstract

Asthma is a chronic inflammatory lung disease that affects 300 million people worldwide. Genetic and environmental factors have been associated with triggering the disease. One od the most environmental factors, it is the exposure to microbes in early life (enviromental factor) that protects against allergies, called as hygiene hypothesis. Since this theory was proposed, many studies have investigated the role of intestinal micro-biota on the modulation of the immune system, and allergy. Studies have shown differences in the composition of the micro-flora between healthy and allergic infants in countries with a high and low prevalence of allergies and between healthy and allergic infants within such countries. Alongside the scientific findings about the importance of the intestinal micro-biota in the prevalence of allergic diseases, probiotics market is expanding with promises of prevention of allergic diseases like asthma. However, it is unclear the effects and mechanisms of probiotic use in the prevention and treatment of asthma. Evidences in the literature also support the possibility that short chain: fatty acids produced by some intestinal bacteria, as acetate, it has anti-inflammatory effects. Moreover, animals deficient GPR43, important receptor to acetate, have an exaggerated inflammatory response models of colitis, arthritis and asthma. The p hypothesis of the present study is that probiotic bacteria could modulate Th2 immune responses via production of short chain fatty acids, such as acetate. We will use two different probiotics, B. longum and B. adolescentis to better investigate the role of short fatty acids produced by probiotics on allergic diseases. The choice of the two species was based on the fact that one is associated with allergy protection and produces more acetate (B. longum) and B. adolescentis it is not associated with allergy protection and it is not a relevant acetate producer. (AU)

Scientific publications

(13)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, CAROLINE MARCANTONIO; VIEIRA, ANGELICA THOMAZ; RAMIREZ VINOLO, MARCO AURELIO; OLIVEIRA, FERNANDO A.; CURI, RUI; MARTINS, FLAVIANO DOS SANTOS. The Central Role of the Gut Microbiota in Chronic Inflammatory Diseases. CLINICAL & DEVELOPMENTAL IMMUNOLOGY, 2014. (12/50336-2, 12/10653-9, 12/50410-8)

MENDES, E.; CASARO, M. B.; FUKUMORI, C.; RIBEIRO, W. R.; DOS SANTOS, A. L.; SARTORELLI, P.; LAZARINI, M.; BOGSAN, C. S. B.; OLIVEIRA, M. A.; FERREIRA, C. M.. Preventive oral kefir supplementation protects mice from ovariectomy-induced exacerbated allergic airway inflammation. BENEFICIAL MICROBES, v. 12, n. 2, p. 187-197, 2021. (12/50410-8, 16/13496-2)

RIBEIRO, W. R.; QUEIROZ, A. G.; MENDES, E.; CASARO, M. B.; NASCIMENTO, C. M.; COELHO, L. S. S. F.; MARTINS, F. S.; LEITE-SILVA, V. R.; FERREIRA, C. M.. Preventive oral supplementation with Bifidobacterium longum 5(1A) alleviates oxazolone-induced allergic contact dermatitis-like skin inflammation in mice. BENEFICIAL MICROBES, v. 12, n. 2, p. 199-209, 2021. (12/50410-8, 19/12324-1)

FERREIRA, CAROLINE M.; WILLIAMS, JESSE W.; TONG, JIANKUN; RAYON, CRYSTAL; BLAINE, KELLY M.; SPERLING, I, ANNE. Allergen Exposure in Lymphopenic Fas-Deficient Mice Results in Persistent Eosinophilia Due to Defects in Resolution of Inflammation. FRONTIERS IN IMMUNOLOGY, v. 9, OCT 30 2018. (12/50410-8)

RIBEIRO, WILLIAN RODRIGUES; RAMIREZ VINOLO, MARCO AURELIO; CALIXTO, LEANDRO AUGUSTO; FERREIRA, CAROLINE MARCANTONIO. Use of Gas Chromatography to Quantify Short Chain Fatty Acids in the Serum, Colonic Luminal Content and Feces of Mice. BIO-PROTOCOL, v. 8, n. 22, NOV 20 2018. (12/50410-8)

MENDES, EDUARDO; ACETTURI, BEATRIZ G.; THOMAS, ANDREW M.; MARTINS, FLAVIANO DOS S.; CRISMA, AMANDA R.; MURATA, GILSON; BRAGA, TARCIOT; CAMARA, NIELS O. S.; FRANCO, ADRIANA L. DOS S.; SETUBAL, JOAO C.; et al. Prophylactic Supplementation of Bifidobacterium longum 5(1A) Protects Mice from Ovariectomy-Induced Exacerbated Allergic Airway Inflammation and Airway Hyperresponsiveness. FRONTIERS IN MICROBIOLOGY, v. 8, SEP 11 2017. (12/50410-8)

FELIZARDO, RAPHAEL J. F.; DE ALMEIDA, DANILO C.; PEREIRA, RAFAEL L.; WATANABE, INGRID K. M.; DOIMO, NAYARA T. S.; RIBEIRO, WILLIAN R.; CENEDEZE, MARCOS A.; HIYANE, MEIRE I.; AMANO, MARIANE T.; BRAGA, TARCIO T.; et al. Gut microbial metabolite butyrate protects against proteinuric kidney disease through epigenetic- and GPR109a-mediated mechanisms. FASEB JOURNAL, v. 33, n. 11, p. 11894-11908, NOV 2019. (14/13135-4, 12/02270-2, 12/15205-4, 12/50410-8, 17/05264-7)

CASARO, M. C.; CRISMA, A. R.; VIEIRA, A. T.; SILVA, G. H. M.; MENDES, E.; RIBEIRO, W. R.; MARTINS, F. S.; FERREIRA, C. M.. Prophylactic Bifidobacterium adolescentis ATTCC 15703 supplementation reduces partially allergic airway disease in Balb/c but not in C57BL/6 mice. BENEFICIAL MICROBES, v. 9, n. 3, p. 465-476, 2018. (12/50410-8)

CASARO, MATEUS B.; THOMAS, ANDREW M.; MENDES, EDUARDO; FUKUMORI, CLAUDIO; RIBEIRO, WILLIAN R.; OLIVEIRA, FERNANDO A.; CRISMA, AMANDA R.; MURATA, GILSON M.; BIZZARRO, BRUNA; SA-NUNES, ANDERSON; et al. A probiotic has differential effects on allergic airway inflammation in A/J and C57BL/6 mice and is correlated with the gut microbiome. MICROBIOME, v. 9, n. 1, JUN 10 2021. (12/50410-8, 16/13496-2, 19/12324-1)

VIEIRA, ANGELICA THOMAZ; FUKUMORI, CLAUDIO; FERREIRA, CAROLINE MARCANTONIO. New insights into therapeutic strategies for gut microbiota modulation in inflammatory diseases. CLINICAL & TRANSLATIONAL IMMUNOLOGY, v. 5, JUN 2016. (12/50410-8)

VIEIRA, ANGELICA T.; CASTELO, PAULA M.; RIBEIRO, DANIEL A.; FERREIRA, CAROLINE M.. Influence of Oral and Gut Microbiota in the Health of Menopausal Women. FRONTIERS IN MICROBIOLOGY, v. 8, SEP 28 2017. (12/50410-8)

FUKUMORI, CLAUDIO; CASARO, MATEUS B.; THOMAS, ANDREW M.; MENDES, EDUARDO; RIBEIRO, WILLIAN R.; CRISMA, AMANDA R.; MURATA, GILSON M.; BIZZARRO, BRUNA; DIAS-NETO, EMMANUEL; SETUBAL, JOAO C.; et al. Maternal supplementation with a synbiotic has distinct outcomes on offspring gut microbiota formation in A/J and C57BL/6 mice, differentially affecting airway inflammatory cell infiltration and mucus production. Journal of Functional Foods, v. 61, OCT 2019. (12/50410-8, 16/13496-2)

FERREIRA, CAROLINE MARCANTONIO; VIEIRA, ANGELICA THOMAZ; RAMIREZ VINOLO, MARCO AURELIO; OLIVEIRA, FERNANDO A.; CURI, RUI; MARTINS, FLAVIANO DOS SANTOS. The Central Role of the Gut Microbiota in Chronic Inflammatory Diseases. JOURNAL OF IMMUNOLOGY RESEARCH, v. 2014, p. 12-pg., 2014-01-01. (12/50410-8, 12/10653-9, 12/50336-2)