Gene expression of MDR-1, TP53, Bcl-2 and Bax in canine transmissible venereal tumor and its relationship to aggression and response to therapy

• Grant number: 12/19285-2
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2013
• End date: May 31, 2015
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Noeme Sousa Rocha
• Grantee: Noeme Sousa Rocha
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Daisy Maria Favero Salvadori ; Glenda Nicioli da Silva

Abstract

The transmissible venereal tumor - TVT along the years has been subject of numerous investigations, nevertheless, there are gaps that need more studies, there are TVTs more malignant, do not respond satisfactorily to conventional therapeutic, and it seem that when these cells exhibit these characteristics, are present and so exacerbated progressive modifications of the biological profile. With the advent of research, such as cell culture, flow cytometry and molecular biology, is possible to identify and quantify these modifications, even predict the biological behavior of this entity. The aim of the study is to quantify the expression of genes BAX, BCL2, TP53 and MDR1 in TVT cells (before culture), and in primary culture of TVT for 3 passages before and after therapy with vincristine that help to identify modifications related with to resistance and tumor malignancy. For both will be used 8 tumors coming of dog, males and females with cytological diagnosis of TVT, seeking to evaluate tests of cytotoxicity, cell survival, growth curve, apoptosis and expression of genes by RT-PCR. It is expected that the results may contribute to understanding the dynamics of tumor growth and its behavior in anticancer treatment. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALZATE, JULIANA M.; MONTOYA-FLOREZ, LUIS M.; PEREZ, JORGE E.; ROCHA, NOEME S.; PEDRAZA-ORDONEZ, FRANCISCO J.. The role of the multi-drug resistance 1, p53, b cell lymphoma 2, and bcl 2-associated X genes in the biologic behavior and chemotherapeutic resistance of canine transmissible venereal tumors. VETERINARY CLINICAL PATHOLOGY, v. 48, n. 4, p. 730-739, DEC 2019. (12/19285-2)

HALINE BALLESTERO FÊO; LUIS MAURICIO MONTOYA FLÓREZ; RICARDO SEITI YAMATOGI; ANDERSON DO PRADO DUZANSKI; JOÃO PESSOA ARAÚJO JUNIOR; ROGERIO ANTONIO DE OLIVEIRA; NOEME SOUSA ROCHA. Alterações nas expressões gênicas influenciam na tumorigênese no tumor venéreo transmissível em cães?. Ciência Rural, v. 50, n. 11, 2020-10-07. (12/19285-2)

HALINE B. FÊO; LUIS MAURICIO M. FLÓREZ; RICARDO S. YAMATOGI; ANDERSON P. DUZANSKI; JOÃO P. ARAÚJO JUNIOR; ROGERIO A. OLIVEIRA; NOEME S. ROCHA. A desregulação da expressão de RASSF1 pode ser um mecanismo da tumorigênese em TVTC?. Pesquisa Veterinária Brasileira, v. 42, 2022-07-29. (12/19285-2)