Characterization of apoptosis related genes in canine cutaneous mast cell tumors and its value as prognostic indicators

• Grant number: 13/13252-8
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2013
• End date: February 29, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Ricardo de Francisco Strefezzi
• Grantee: Ricardo de Francisco Strefezzi
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Associated researchers: Heidge Fukumasu ; José Guilherme Xavier ; Jose Luiz Catao Dias ; Maria Lucia Zaidan Dagli ; Silvia Regina kleeb

Abstract

Mast cell tumors are potentially malignant neoplasms which have variable macroscopic features and biological behavior. These factors have turned very difficult the formulation of accurate prognosis and treatment. Alterations of apoptosis favor tumor progression by allowing the survival of the neoplastic cells in the circulation and other microenvironments. The pro-apoptotic protein BAX forms nonselective pores in the outer mitochondrial membrane. This phenomenon initiates the release of cytochrome C into the cytoplasm and activates Apaf-1 and the caspases 3 and 9, resulting in cell death. On the other hand, BCL2, an anti-apoptotic protein, antagonizes the effects of BAX and prolongs the life of the cell. The objective of this study is to detect and quantify the protein expression of BAX, BCL2, Apaf-1, caspase 3 and caspase 9, as well as their respective m-RNA in canine cutaneous mast cell tumors and to correlate these with the histopathological grading, clinical evolution and mortality. Thus, one hundred cases of canine cutaneous mast cell tumors will be submitted to histological, immunohistochemical and molecular analyzes. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VARGAS, T. H. M.; PULZ, L. H.; FERRO, D. G.; SOBRAL, R. A.; VENTURINI, M. A. F. A.; CORREA, H. L.; STREFEZZI, R. F.. Galectin-3 Expression Correlates with Post-surgical Survival in Canine Oral Melanomas. Journal of Comparative Pathology, v. 173, p. 49-57, NOV 2019. (13/13252-8, 15/11959-2, 16/03862-1)

DANIEL, JESSIKA; BARRA, CAMILA N.; PULZ, LIDIA H.; KLEEB, SILVIA REGINA; XAVIER, JOSE GUILHERME; CATAO-DIAS, JOSE LUIZ; NISHIYA, ADRIANA T.; FUKUMASU, HEIDGE; STREFEZZI, RICARDO F.. Intratumoral collagen index predicts mortality and survival in canine cutaneous mast cell tumours. VETERINARY DERMATOLOGY, v. 30, n. 2, p. 162+, APR 2019. (13/13252-8, 16/03862-1, 10/05094-5)

VARGAS, T. H. M.; PULZ, L. H.; BARRA, C. N.; KLEEB, S. R.; XAVIER, J. G.; CATAO-DIAS, J. L.; FUKUMASU, H.; NISHIYA, A. T.; STREFEZZI, R. F.. Immunohistochemical Expression of the Pluripotency Factor OCT4 in Canine Mast Cell Tumours. Journal of Comparative Pathology, v. 153, n. 4, p. 251-255, NOV 2015. (10/05094-5, 13/13252-8)

VARGAS, THIAGO HENRIQUE M.; BARRA, CAMILA N.; PULZ, LIDIA H.; HUETE, GREICE C.; CADROBBI, KARINE G.; NISHIYA, ADRIANA TOMOKO; KLEEB, SILVIA REGINA; XAVIER, JOSE GUILHERME; CATAO-DIAS, JOSE LUIZ; STREFEZZI, RICARDO F.. Galectin-3 immunolabelling correlates with BCL2 expression in canine cutaneous mast cell tumours. ACTA VETERINARIA HUNGARICA, v. 69, n. 2, p. 169-174, JUN 2021. (13/13252-8, 15/11959-2, 16/03862-1)

BARRA, C. N.; MACEDO, B. M.; CADROBBI, K. G.; PULZ, L. H.; HUETE, G. C.; KLEEB, S. R.; XAVIER, J. G.; CATAO-DIAS, J. L.; NISHIYA, A. T.; FUKUMASU, H.; et al. Apoptotic intrinsic pathway proteins predict survival in canine cutaneous mast cell tumours. VETERINARY AND COMPARATIVE ONCOLOGY, v. 16, n. 1, p. E38-E44, MAR 2018. (13/13252-8, 16/03862-1)

PULZ, LIDIA H.; BARRA, CAMILA N.; ALEXANDRE, PAMELA A.; HUETE, GREICE C.; CADROBBI, KARINE G.; NISHIYA, ADRIANA T.; DE FREITAS, SILVIO HENRIQUE; FUKUMASU, HEIDGE; STREFEZZI, RICARDO F.. Identification of two molecular subtypes in canine mast cell tumours through gene expression profiling. PLoS One, v. 14, n. 6, JUN 19 2019. (13/13252-8, 14/02493-7)

JOSELEVITCH, JULIA A.; BARRA, CAMILA N.; VARGAS, THIAGO HENRIQUE M.; PULZ, LIDIA H.; NISHIYA, ADRIANA T.; KLEEB, SILVIA REGINA; XAVIER, JOSE GUILHERME; CATAO-DIAS, JOSE LUIZ; STREFEZZI, RICARDO F.. Nanog Expression and Proliferation Indices in Canine Cutaneous Mast Cell Tumors. VETERINARY PATHOLOGY, v. 55, n. 6, p. 849-852, NOV 2018. (13/13252-8, 14/20872-5, 16/03862-1)

PULZ, L. H.; BARRA, C. N.; KLEEB, S. R.; XAVIER, J. G.; CATAO-DIAS, J. L.; SOBRAL, R. A.; FUKUMASU, H.; STREFEZZI, R. F.. Increased expression of tissue inhibitor of metalloproteinase-1 correlates with improved outcome in canine cutaneous mast cell tumours. VETERINARY AND COMPARATIVE ONCOLOGY, v. 15, n. 2, p. 606-614, JUN 2017. (10/05094-5, 13/13252-8)

PULZ, L. H.; STREFEZZI, R. F.. Proteases as prognostic markers in human and canine cancers. VETERINARY AND COMPARATIVE ONCOLOGY, v. 15, n. 3, p. 669-683, SEP 2017. (10/05094-5, 13/13252-8)

MARQUES, G. R.; ROCHA, L. F.; VARGAS, T. H. M.; PULZ, L. H.; HUETE, G. C.; CADROBBI, K. G.; PIRES, C. G.; SANCHES, D. S.; MOTA, E. F. F.; STREFEZZI, R. F.. Relationship of Galectin-3 Expression in Canine Cutaneous Squamous Cell Carcinomas with Histopathological Grading and Proliferation Indices. Journal of Comparative Pathology, v. 178, p. 16-21, JUL 2020. (13/13252-8, 16/16239-0, 16/03862-1)