Performance of methods for diagnosis of multidrug-resistant tuberculosis and Extensively Resistant. Applicability in a Reference Laboratory

• Grant number: 12/51756-5
• Support Opportunities: Research Grants - Research in Public Policies for the National Health Care System (PP-SUS)
• Start date: July 01, 2013
• End date: December 31, 2015
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Agreement: CNPq - PPSUS
• Principal Investigator: Lucilaine Ferrazoli
• Grantee: Lucilaine Ferrazoli
• Host Institution: Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

According to the World Health Organization, 6.2 million cases of tuberculosis (TB) were diagnosed and reported worldwide in 2010, with 5.4 million new cases. Brazil is among the 22 countries which account for 82% of TB cases in the world. Although the incidence rate has decreased from 42.8/100,000 inhabitants in 2001 to 36.0/100,000 inhabitants in 2011, TB is considered a serious disease in Brazil. Drug resistance to TB threatens the control of this disease in the word. New and faster diagnostic tests to detect drug resistance have been developed to improve the effective treatment of TB. The GenoType MTBDRplus (Hain LifeScience, Germany) is a rapid molecular test that simultaneously detects M. tuberculosis complex and mutations in genes rpoB, katG and inhA, responsible for resistance against isoniazid and rifampicin. The GenoType MTBDRsl test (Hain LifeScience, Germany) detects resistance to fluoroquinolones, aminoglycosides/cyclic peptides, and ethambutol, therefore identifying extended resistance. The objective of this study is to evaluate the accuracy of both tests by submitting 306 cultures of M. tuberculosis complex isolates to the GenoType MTBDRplus and 196 isolates to the GenoType MTBDRsl test. The MGIT 960 method, which is the gold standard method to determine TB is routinely used in the Core of Tuberculosis and Mycobacteriosis of Instituto Adolfo Lutz and will be the reference test. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

WATANABE PINHATA, JULIANA MAIRA; BRANDAO, ANGELA PIRES; MENDES, FLAVIA DE FREITAS; DA SILVA RABELLO, MICHELLE CHRISTIANE; FERRAZOLI, LUCILAINE; DE OLIVEIRA, ROSANGELA SIQUEIRA. Correlating genetic mutations with isoniazid phenotypic levels of resistance in Mycobacterium tuberculosis isolates from patients with drug-resistant tuberculosis in a high burden setting. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, v. 40, n. 12, JUL 2021. (12/51756-5, 17/16082-7)

FOWLER, PHILIP W.; BARILAR, IVAN; BATTAGLIA, SIMONE; BORRONI, EMANUELE; BRANDAO, ANGELA PIRES; BRANKIN, ALICE; CABIBBE, ANDREA MAURIZIO; CARTER, JOSHUA; CIRILLO, DANIELA MARIA; CLAXTON, PAULINE; et al. Epidemiological cut-off values for a 96-well broth microdilution plate for high-throughput research antibiotic susceptibility testing of M. tuberculosis. European Respiratory Journal, v. 60, n. 4, p. 22-pg., 2022-10-01. (17/16082-7, 12/51756-5)

[ANONYMOUS]. A data compendium associating the genomes of 12,289 Mycobacterium tuberculosis isolates with quantitative resistance phenotypes to 13 antibiotics. PLOS BIOLOGY, v. 20, n. 8, p. 25-pg., 2022-08-01. (12/51756-5, 17/16082-7)

RODRIGUES, CAMILLA; MOORE, DAVID; CROOK, DERRICK W.; CIRILLO, DANIELA M.; FOWLER, PHILIP W.; IQBAL, ZAMIN; ISMAIL, NAZIR A.; MISTRY, NERGES; NIEMANN, STEFAN; PETO, TIM E. A.; et al. Genome-wide association studies of global Mycobacterium tuberculosis resistance to 13 antimicrobials in 10,228 genomes identify new resistance mechanisms. PLOS BIOLOGY, v. 20, n. 8, p. 27-pg., 2022-08-01. (12/51756-5, 17/16082-7)

BRANDAO, ANGELA PIRES; WATANABE PINHATA, JULIANA MAIRA; SIMONSEN, VERA; OLIVEIRA, ROSANGELA SIQUEIRA; GHISI, KELEN TEIXEIRA; SILVA RABELLO, MICHELLE CHRISTIANE; FUKASAVA, SUELY; FERRAZOLI, LUCILAINE. Transmission of Mycobacterium tuberculosis presenting unusually high discordance between genotypic and phenotypic resistance to rifampicin in an endemic tuberculosis setting. TUBERCULOSIS, v. 125, DEC 2020. (17/16082-7, 12/51756-5)

ANGELA PIRES BRANDAO; JULIANA MAIRA WATANABE PINHATA; ROSANGELA SIQUEIRA OLIVEIRA; VERA MARIA NEDER GALESI; HELIO HEHL CAIAFFA-FILHO; LUCILAINE FERRAZOLI. Agilizando o diagnóstico da tuberculose multirresistente em uma região endêmica com o uso de um teste comercial de sondas em linha. Jornal Brasileiro de Pneumologia, v. 45, n. 2, 2019-04-18. (12/51756-5)

WATANABE PINHATA, JULIANA MAIRA; BRANDAO, ANGELA PIRES; GALLO, JULIANA FAILDE; SIQUEIRA DE OLIVEIRA, ROS ANGELA; FERRAZOLI, LUCILAINE. GenoType MTBDRsl for detection of second-line drugs and ethambutol resistance in multidrug-resistant Mycobacterium tuberculosis isolates at a high-throughput laboratory. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 105, n. 2, p. 9-pg., 2023-02-01. (12/51756-5, 17/16082-7)