Proteomics analyses of meso and thermophilic filamentous fungi exposed to physical and chemical factors

• Grant number: 12/24703-8
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2014
• End date: September 30, 2016
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Principal Investigator: Hamilton Cabral
• Grantee: Hamilton Cabral
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The proteome is the complete set of proteins encoded by the genome and allows to understand how cells adapt to different environmental conditions and provides important information about the proteins quantities and post-translational modifications.The proteomic studies have become an excellent tool to explore, discover and identify the proteins expressed in response to environmental stress, such as carbon source, temperature and pH, because these parameters affect the production of microbial enzymes. Understanding the mechanisms of production and secretion of proteins can be assisted by proteomics and elucidation of these steps may improve the results obtained in bioprocesses. This project intends to continue several subprojects involving prospecting research line of hydrolytic enzymes from microbial source and the enzymes that are targets of this research are proteases, carbohydrases and lipases. The objective of this project is to investigate, using proteomics techniques, the behavior and the enzymes production of the meso and thermophilic filamentous fungi when exposed to different physical and chemical factors. The biochemical potential study of protein expression of each fungus is evaluated by different factors, such as carbon and nitrogen sources, effect of pH and temperature among others. The analyses will be performed by 1D and 2D gels, and the spots differentially expressed in comparison to standard medium will be removed and held on trypsin digestion. The obtained fragments will be applied in the mass spectrometer to obtain the peptides sequences which will be analyzed in silico. The studies on protein expression in filamentous fungi under different conditions of growth open the possibility of exploiting enzymes not yet identified or detected by conventional techniques. Also is possible to discover the best conditions to optimize the enzymes production, considering the biochemical behavior of each fungus in variations of solid and submerged bioprocesses. Thus, the proteomic analyses allow the detection and identification of different proteins expressed in different conditions of microbial growth, contributing to identification of sets protein. (AU)

Scientific publications

(18)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, RONIVALDO RODRIGUES; SOUTO, TATIANE BELTRAMINI; DA ROSA, NATHALIA GONSALES; GONCALVES DE OLIVEIRA, LILIAN CAROLINE; JULIANO, MARIA APARECIDA; JULIANO, LUIZ; ROSA, JOSE C.; CABRAL, HAMILTON. Evaluation of the milk clotting properties of an aspartic peptidase secreted by Rhizopus microsporus. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, OCT 2019. (11/06986-0, 12/24703-8)

HAMIN NETO, YOUSSEF A. A.; DE OLIVEIRA, LILIAN C. G.; DE OLIVEIRA, ARTHUR H. C.; ROSA, JOSE C.; JULIANO, MARIA A.; JULIANO, LUIZ; RODRIGUES, ANDRE; CABRAL, HAMILTON. Determination of Specificity and Biochemical Characteristics of Neutral Protease Isolated from Myceliophthora thermophila. PROTEIN AND PEPTIDE LETTERS, v. 22, n. 11, p. 972-982, 2015. (12/50191-4, 12/18278-2, 11/06986-0, 12/24703-8)

COITINHO, LUCIANA BARBOSA; FUMAGALLI, FERNANDO; DA ROSA-GARZON, NATHALIA GONSALES; EMERY, FLAVIO DA SILVA; CABRAL, HAMILTON. Lapachol biotransformation by filamentous fungi yields bioactive quinone derivatives and lapachol-stimulated secondary metabolites. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, v. 49, n. 5, p. 459-463, MAY 28 2019. (14/01497-9, 12/24703-8)

DA ROSA-GARZON, NATHALIA GONSALES; LAURE, HELEN JULIE; DE SOUZA-MOTTA, CRISTINA MARIA; ROSA, JOSE CESAR; CABRAL, HAMILTON. Medium pH in submerged cultivation modulates differences in the intracellular protein profile of Fusarium oxysporum. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, v. 47, n. 7, p. 664-672, 2017. (12/24703-8)

HAMIN NETO, YOUSSEF A. A.; DE OLIVEIRA, LILIAN C. G.; DE OLIVEIRA, JULIANA R.; JULIANO, MARIA A.; JULIANO, LUIZ; ARANTES, ELIANE C.; CABRAL, HAMILTON. Analysis of the Specificity and Biochemical Characterization of Metalloproteases Isolated from Eupenicillium javanicum Using Fluorescence Resonance Energy Transfer Peptides. FRONTIERS IN MICROBIOLOGY, v. 7, JAN 9 2017. (11/06986-0, 12/24703-8)

DA ROSA-GARZON, NATHALIA GONSALES; LAURE, HELEN JULIE; ROSA, JOSE CESAR; CABRAL, HAMILTON. Valorization of agricultural residues using Myceliophthora thermophila as a platform for production of lignocellulolytic enzymes for cellulose saccharification. BIOMASS & BIOENERGY, v. 161, p. 9-pg., 2022-04-25. (12/24703-8)

DA SILVA, RONIVALDO RODRIGUES; SOUTO, TATIANE BELTRAMINI; DA ROSA, NATHALIA GONSALES; GONCALVES DE OLIVEIRA, LILIAN CAROLINE; JULIANO, MARIA APARECIDA; JULIANO, LUIZ; ROSA, JOSE C.; CABRAL, HAMILTON. Evaluation of the milk clotting properties of an aspartic peptidase secreted by Rhizopus microsporus. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, v. 50, n. 3, p. 8-pg., 2019-10-26. (11/06986-0, 12/24703-8)

DA ROSA-GARZON, NATHALIA GONSALES; LAURE, HELEN JULIE; ROSA, JOSE CESAR; CABRAL, HAMILTON. Fusarium oxysporum cultured with complex nitrogen sources can degrade agricultural residues: Evidence from analysis of secreted enzymes and intracellular proteome. RENEWABLE ENERGY, v. 133, p. 941-950, APR 2019. (12/24703-8)

DA SILVA, RONIVALDO RODRIGUES; GONCALVES DE OLIVEIRA, LILIAN CAROLINE; JULIANO, MARIA APARECIDA; JULIANO, LUIZ; ROSA, JOSE C.; CABRAL, HAMILTON. Activity of a peptidase secreted by Phanerochaete chrysosporium depends on lysine to subsite S `(1). International Journal of Biological Macromolecules, v. 94, n. A, p. 474-483, JAN 2017. (11/06986-0, 12/24703-8)

YOUSSEF ALI ABOU HAMIN NETO; NATHÁLIA GONSALES DA ROSA GARZON; LUCIANA BARBOSA COITINHO; LAYS MARTIN SOBRAL; ANDRÉIA MACHADO LEOPOLDINO; THAÍS REGIANI CATALDI; CARLOS ALBERTO LABATE; HAMILTON CABRAL. Fungal metalloprotease generate whey-derived peptides that may be involved in apoptosis in B16F10 melanoma cells. FOOD SCIENCE AND TECHNOLOGY, v. 42, 2022-07-26. (12/18278-2, 20/14426-3, 12/24703-8)

DA SILVA, RONIVALDO RODRIGUES; DA ROSA, NATHALIA GONSALES; GONCALVES DE OLIVEIRA, LILIAN CAROLINE; JULIANO, MARIA APARECIDA; JULIANO, LUIZ; ROSA, JOSE C.; CABRAL, HAMILTON. Biochemical Properties and Catalytic Specificity of a Novel Neutral Serine Peptidase Secreted by Fungus Pyrenochaetopsis sp.. Applied Biochemistry and Biotechnology, v. 187, n. 4, p. 1158-1172, APR 2019. (11/06986-0, 12/24703-8)

DA SILVA, RONIVALDO RODRIGUES; GONCALVES DE OLIVEIRA, LILIAN CAROLINE; JULIANO, MARIA APARECIDA; JULIANO, LUIZ; DE OLIVEIRA, ARTHUR H. C.; ROSA, JOSE C.; CABRAL, HAMILTON. Biochemical and milk-clotting properties and mapping of catalytic subsites of an extracellular aspartic peptidase from basidiomycete fungus Phanerochaete chrysosporium. Food Chemistry, v. 225, p. 45-54, JUN 15 2017. (12/24703-8, 11/06986-0)

IDA, ERIKA LIKA; DA SILVA, RONIVALDO RODRIGUES; DE OLIVEIRA, TASSIO BRITO; SOUTO, TATIANE BELTRAMINI; LEITE, JULIANA ABIGAIL; RODRIGUES, ANDRE; CABRAL, HAMILTON. Biochemical properties and evaluation of washing performance in commercial detergent compatibility of two collagenolytic serine peptidases secreted by Aspergillus fischeri and Penicillium citrinum. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, v. 47, n. 3, p. 282-290, 2017. (11/06986-0, 12/24703-8)

BIAGGIO, RAFAEL TAGE; DA SILVA, RONIVALDO RODRIGUES; DA ROSA, NATHALIA GONSALES; RIBEIRO LEITE, RODRIGO SIMOES; ARANTES, ELIANE CANDIANI; DE FREITAS CABRAL, TATIANA PEREIRA; JULIANO, MARIA A.; JULIANO, LUIZ; CABRAL, HAMILTON. Purification and biochemical characterization of an extracellular serine peptidase from Aspergillus terreus. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, v. 46, n. 3, p. 298-304, 2016. (11/06986-0, 12/24703-8)

DA SILVA, RONIVALDO RODRIGUES; SOUTO, TATIANE BELTRAMINI; DE OLIVEIRA, TASSIO BRITO; GONCALVES DE OLIVEIRA, LILIAN CAROLINE; KARCHER, DANIEL; JULIANO, MARIA APARECIDA; JULIANO, LUIZ; DE OLIVEIRA, ARTHUR H. C.; RODRIGUES, ANDRE; ROSA, JOSE C.; et al. Evaluation of the catalytic specificity, biochemical properties, and milk clotting abilities of an aspartic peptidase from Rhizomucor miehei. Journal of Industrial Microbiology & Biotechnology, v. 43, n. 8, p. 1059-1069, AUG 2016. (11/06986-0, 12/24703-8)

ABOU HAMIN NETO, YOUSSEF ALI; COITINHO, LUCIANA BARBOSA; PEDRO DE FREITAS, LUIS ALEXANDRE; CABRAL, HAMILTON. Box-Behnken analysis and storage of spray-dried collagenolytic proteases from Myceliophthora thermophila submerged bioprocess. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY, v. 47, n. 5, p. 473-480, 2017. (11/06986-0, 12/18278-2, 12/24703-8)

LEITE, JULIANA ABIGAIL; GONSALES DA ROSA-GARZON, NATHALIA; LAURE, HELEN JULIE; ROSA, JOSE CESAR; FRANCO, OCTAVIO LUIZ; DE SOUZA MOTTA, CRISTINA MARIA; CABRAL, HAMILTON. Proteomic Analysis of Intra- and Extracellular Proteins of Aspergillus Niveus During Submerged Bioprocess Culturing Under Different pH Con- ditions. CURRENT PROTEOMICS, v. 18, n. 4, p. 563-574, 2021. (12/24703-8)

DA SILVA, RONIVALDO RODRIGUES; GONCALVES DE OLIVEIRA, LILIAN CAROLINE; JULIANO, MARIA APARECIDA; JULIANO, LUIZ; ROSA, JOSE C.; CABRAL, HAMILTON. Activity of a peptidase secreted by Phanerochaete chrysosporium depends on lysine to subsite S '(1). International Journal of Biological Macromolecules, v. 94, p. 10-pg., 2017-01-01. (11/06986-0, 12/24703-8)