Longitudinal analysis of the subgingival microbiome following enamel matrix derivate therapy

• Grant number: 13/50389-1
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2013
• End date: September 30, 2014
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Agreement: Ohio State University
• Principal Investigator: Renato Corrêa Viana Casarin
• Grantee: Renato Corrêa Viana Casarin
• Principal researcher abroad: Purmina Kumar
• Institution abroad: Ohio State University, Columbus, United States
• Host Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil
• Associated research grant: 12/50877-3 - Evaluation of locally applied 2% doxiciclin PLGA nanosphers as adjunctive therapy in the treatment of chronic periodontitis in type-2 Diabetes mellitus individuals: clinical, micro and immunological analysis, AP.R

Abstract

Periodontitis is a polymicrobial infection that destroys the structures anchoring the tooth to the jawbone and results in eventual tooth loss. This disease affects 65% of individuals over 40 years of age and has been consistently associated with several potentially life-threatening diseases including coronary heart disease, pre-term births, diabetes and cerebrovascular accidents. Subgingival bacteria are the primary etiologic agents of periodontitis. Evidence is emerging that Enamel Matrix Derivative (EMD) is capable of regenerating lost periodontal tissues both by stimulating stem cells within the periodontal ligament and through an antimicrobial activity. Although the regenerative mechanisms of EMD have been well elucidated, the antimicrobial effects have been poorly characterized. Recent studies, including our own investigations, have shown that more than 70% of the subgingival microbiome is uncultivated and therefore, unrecognized. Accordingly, a comprehensive examination of this complex ecosystem requires an approach that would identify all species in the community, including previously uncultivated, unknown and unsuspected organisms. We propose studies that combine a randomized clinical study design with high-throughput molecular microbiology to elucidate the effects of EMD on subgingival bacteria. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

QUEIROZ, LUCAS A.; CASARIN, RENATO C. V.; DABDOUB, SHAREEF M.; TATAKIS, DIMITRIS N.; SALLUM, ENILSON A.; KUMAR, PURNIMA S.. Furcation Therapy With Enamel Matrix Derivative: Effects on the Subgingival Microbiome. Journal of Periodontology, v. 88, n. 7, p. 617-625, JUL 2017. (13/50389-1)