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Effect of leukocytes colostrum mother in immune response of newborn calves

Grant number: 13/06152-7
Support Opportunities:Regular Research Grants
Duration: May 01, 2014 - April 30, 2016
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal Investigator:Viviani Gomes
Grantee:Viviani Gomes
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated researchers: David John Hurley ; Erika Cosendey Toledo de Mello Peixoto


The objective of this project is to evaluate the effect of passive transfer of leukocytes from bovine colostrum in systemic immunity in newborn calves from birth to 28 days of their life. Will be used 20 calves divided into two experimental groups. The calves receive colostrum COL + group with fresh cells from their mothers; animals COL - group receive acellular colostrum. The acellular colostrum is obtained from a "pool", which is centrifuged to remove cells and frozen at -80 °C. Calves will be assessed at the following times: before feeding colostrum, 24 hours, 2 days (d), 7d, 14d, 21d and 28d after birth. Clinical examination of newborns will be performed and blood samples will be collected into tubes with EDTA (4mL) and anticoagulant heparin (40mL). The innate immune response will be assessed by tests of phagocytosis and release of free radicals by blood monocytes and polymorphonuclear by stimulating bacterial. Specific immunity will be verified by the cultivation of blood lymphocytes and their production of cytokines interferon-gamma (cell response - Th1) and interleukin-10 (humoral response - Th2) stimulated with inactivated bovine herpesvirus (BoHV). Furthermore, immunophenotyping tests will be performed to determine the proportion of the types of leukocytes and their activation. The results obtained using these variables will be tested using the Kolmogorov-Smirnov normality. If they present parametric distribution will be subjected to analysis of variance and differences assessed by Duncan test (SAMPAIO, 1998). The results that do not show this distribution will be tested using the Mann-Whitney test (SIEGEL, 1975). (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARTIN, CAMILA CECILIA; FONTES NOVO DE OLIVEIRA, SYLVIA MARQUART; DOS REIS COSTA, JULIANA FRANCA; BACCILI, CAMILA COSTA; SILVA, BRUNO TOLEDO; HURLEY, DAVID JOHN; GOMES, VIVIANI. Influence of feeding fresh colostrum from the dam or frozen colostrum from a pool on indicator gut microbes and the inflammatory response in neonatal calves. Research in Veterinary Science, v. 135, p. 355-365, . (13/06152-7)
DOS REIS COSTA, JULIANA FRANCA; FONTES NOVO, SYLVIA MARQUART; BACCILI, CAMILA COSTA; SOBREIRA, NATALIA MEIRELLES; HURLEY, DAVID JOHN; GOMES, VIVIANI. Innate immune response in neonate Holstein heifer calves fed fresh or frozen colostrum. Research in Veterinary Science, v. 115, p. 54-60, . (13/06152-7)
FONTES NOVO, SYLVIA MARQUART; DOS REIS COSTA, JULIANA FRACA; BACCILI, CAMILA COSTA; SOBREIRA, NATALIA MEIRELLES; SILVA, BRUNO TOLEDO; DE OLIVEIRA, PAMELLA LORENCI; HURLEY, DAVID JOHN; GOMES, VIVIANI. Effect of maternal cells transferred with colostrum on the health of neonate calves. Research in Veterinary Science, v. 112, p. 97-104, . (13/02484-5, 13/06152-7)

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