Genetic characterization, sex control, and germ cell transplantation in salmonids stocks from Estação Experimental de Salmonicultura de Campos do Jordão

• Grant number: 13/17612-9
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: April 01, 2014
• End date: March 31, 2019
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Ricardo Shohei Hattori
• Grantee: Ricardo Shohei Hattori
• Host Institution: APTA Regional. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
• Associated researchers: Neuza Sumico Takahashi ; Renata Guimarães Moreira Whitton ; Yara Aiko Tabata
• Associated research grant(s): 18/20200-8 - Involvement of thyroid hormones on the proliferation, differentiation, and depletion of germ cells by high temperature, AV.EXT; 15/50465-5 - Endocrine-disrupting chemicals, global warming and reproductive health in fishes, AP.R SPRINT; 14/50790-0 - Molecular regulation of germ cell proliferation: the effects of thermal stress and sex steroids and the roles of PEN-2, p53 and AMH genes, AP.R SPRINT
• Associated scholarship(s): 14/00569-6 - Caracterização genética, controle de sexo e transplantes de células germinativas em estoques cultivados de salmonídeos da Estação Experimental de Salmonicultura de Campos do Jordão, BP.JP

Abstract

Fish farming of salmonids in Brazil is performed in mountain areas of South-South Eastern Region and the production is mainly based on the rainbow trout Onchorhynchus mykiss. The domestic production is relatively small when compared to large producers like Chile and Norway and is destined for the supplying of local restaurants and hotels. The Estação Experimental de Salmonicultura de Campos do Jordão has several rainbow trout strains with specific traits for each production system or purpose. The application of triploidization, hybridization, and sex control techniques in those strains has led to significant optimization in the production of fertilized eggs/larvae with better traits for local farmers. However, the high demand of fertilized eggs and larvae in addition to the development of new products like the trout salmon and trout caviar, it became necessary to develop new strains, optimize the production of the current ones, and direct the stock breeding using genetic markers in order to attend the high demand for this product. Within this context, the present project was designed to (1) develop genetic markers for distinguishing genetically each strain as well as for the monitoring of their genetic pool, to (2) optimize the production of XXB animals using the sex determining gene of salmonids sdY for the screening of hormonally sex-reversed fish, and to (3) develop protocols for the application of surrogate broodstock approach using different strains of salmonids. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

HATTORI, RICARDO SHOHEI; YOSHINAGA, TULIO TERUO; BUTZGE, ARNO JULIANO; HATTORI-IHARA, SHOKO; TSUKAMOTO, RICARDO YASUICHI; TAKAHASHI, NEUZA SUMICO; TABATA, YARA AIKO. Generation of a white-albino phenotype from cobalt blue and yellow-albino rainbow trout (Oncorhynchus mykiss): Inheritance pattern and chromatophores analysis. PLoS One, v. 15, n. 1, JAN 27 2020. (13/17612-9, 14/00569-6)

YOSHINAGA, TULIO TERUO; KFOURY JUNIOR, JOSE ROBERTO; BUTZGE, ARNO JULIANO; OLIO, RENNAN LOPES; HERNANDEZ-BLAZQUEZ, FRANCISCO JAVIER; OLIVEIRA CARREIRA, ANA CLAUDIA; MASSOCO SALLES GOMES, CRISTINA DE OLIVEIRA; FARIA DA CUNHA BIANCHI, PEDRO KASTEIN; TABATA, YARA AIKO; HATTORI, RICARDO SHOHEI. Testicular subcutaneous allografting followed by immunosuppressive treatment promotes maintenance of spermatogonial cells in rainbow trout (Oncorhynchus mykiss). FISH & SHELLFISH IMMUNOLOGY, v. 112, p. 8-pg., 2021-03-18. (13/17612-9, 15/20762-8)

YOSHINAGA, TULIO TERUO; KFOURY JUNIOR, JOSE ROBERTO; BUTZGE, ARNO JULIANO; OLIO, RENNAN LOPES; HERNANDEZ-BLAZQUEZ, FRANCISCO JAVIER; OLIVEIRA CARREIRA, ANA CLAUDIA; MASSOCO SALLES GOMES, CRISTINA DE OLIVEIRA; FARIA DA CUNHA BIANCHI, PEDRO KASTEIN; TABATA, YARA AIKO; HATTORI, RICARDO SHOHEI. esticular subcutaneous allografting followed by immunosuppressive treatment promotes maintenance of spermatogonial cells in rainbow trout (Oncorhynchus mykiss. FISH & SHELLFISH IMMUNOLOGY, v. 112, p. 108-115, MAY 2021. (15/20762-8, 13/17612-9)

BUTZGE, ARNO JULIANO; YOSHINAGA, TULIO TERUO; ACOSTA, OMAR DAVID MORENO; FERNANDINO, JUAN IGNACIO; SANCHES, EDUARDO ANTONIO; TABATA, YARA AIKO; DE OLIVEIRA, CLAUDIO; TAKAHASHI, NEUZA SUMICO; HATTORI, RICARDO SHOHEI. Early warming stress on rainbow trout juveniles impairs male reproduction but contrastingly elicits intergenerational thermotolerance. SCIENTIFIC REPORTS, v. 11, n. 1, AUG 23 2021. (13/17612-9)

HATTORI, RICARDO SHOHEI; YOSHINAGA, TULIO TERUO; KATAYAMA, NAOTO; HATTORI-IHARA, SHOKO; TSUKAMOTO, RICARDO YASUICHI; TAKAHASHI, NEUZA SUMICO; TABATA, YARA AIKO. Surrogate production of Salmo salar oocytes and sperm in triploid Oncorhynchus mykiss by germ cell transplantation technology. Aquaculture, v. 506, p. 238-245, MAY 15 2019. (13/17612-9)

SARIDA, MUNTI; HATTORI, RICARDO S.; ZHANG, YAN; YAMAMOTO, YOJI; STRUESSMANN, CARLOS A.. Spatiotemporal Correlations between amh and cyp19a1a Transcript Expression and Apoptosis during Gonadal Sex Differentiation of Pejerrey, Odontesthes bonariensis. SEXUAL DEVELOPMENT, v. 13, n. 2, p. 99-107, 2019. (13/17612-9)

GARCIA-CRUZ, ESTEFANY L.; YAMAMOTO, YOJI; HATTORI, RICARDO S.; DE VASCONCELOS, LARISSA MONTEIRO; YOKOTA, MASASHI; STRUSSMANN, CARLOS A.. Crowding stress during the period of sex determination causes masculinization in pejerrey Odontesthes bonariensis, a fish with temperature-dependent sex determination. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 245, JUL 2020. (13/17612-9)

STRUSSMANN, CARLOS A.; YAMAMOTO, YOJI; HATTORI, RICARDO S.; FERNANDINO, I, JUAN; SOMOZA, GUSTAVO M.. Where the Ends Meet: An Overview of Sex Determination in Atheriniform Fishes. SEXUAL DEVELOPMENT, v. 15, n. 1-3, p. 80-92, SEP 2021. (13/17612-9)