Molecular characterization of Staphylococcus sp. isolated from milk of cows with mastitis in different regions in São Paulo State

• Grant number: 13/12831-4
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2014
• End date: February 29, 2016
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Vera Lúcia Mores Rall
• Grantee: Vera Lúcia Mores Rall
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Helio Langoni

Abstract

Staphylococcus sp is common agent of bovine mastitis, causing huge economic losses in Brazilian cattle. This micro-organism posses virulence factors well known as the production of hemolysin, leucotoxinas and toxin superantigens such as toxic shock syndrome and enterotoxins. In addition, several Staphylococcus species can acquire antibiotic resistance genes to lactam antimicrobials. The objective of the study is to characterize molecularly Staphylococcus sp from milk of cows with clinical and subclinical mastitis in various regions of the state of São Paulo, by MLST, spatyping, agr and clonal complex (CC). Also will be performed phenotypic and genotypic tests for antimicrobial resistance (including the SCCmec) and molecular research of some virulence factors such as Panton Valentine toxin, toxic shock syndrome and classical enterotoxins and sec, seh, s9ei, in order to check which virulence factors involved in mastitis disease. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BONSAGLIA, E. C. R.; SILVA, N. C. C.; RUSSI, B. F.; CAMARGO, C. H.; DANTAS, S. T. A.; LANGONI, H.; GUIMARAES, F. F.; LIMA, F. S.; FITZGERALD, J. R.; FERNANDES JUNIOR, A.; et al. Molecular epidemiology of methicillin-susceptible Staphylococcus aureus (MSSA) isolated from milk of cows with subclinical mastitis. Microbial Pathogenesis, v. 124, p. 130-135, NOV 2018. (13/12831-4, 13/18338-8)