Correlation between concentration of waste anesthetic gases with oxidative stress and immune response in medical residents occupationally exposed

• Grant number: 13/21130-0
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2014
• End date: November 30, 2016
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Mariana Gobbo Braz
• Grantee: Mariana Gobbo Braz
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Chung-Yen Chen ; Leandro Gobbo Braz

Abstract

Differently from developed countries, Brazil has no politics of prevention of waste anesthetic gases or studies performed in biomonitoring. Thus, this proposal aims to correlate the concentrations of waste anesthetic gases in the operating rooms (OR) without adequated scavenging air system, what is common in our country, with possible toxic effects in recently exposed medical residents. The study will be performed in the Hospital of Botucatu Medical School - UNESP and in 40 medical residents: 20 without occupational exposure and 20 residents of anesthesiology and surgery with three years of exposure to waste anesthetic gases. The concentrations of isoflurane, sevoflurane, desflurane and nitrous oxide (waste anesthetic gases) will be determined in the operating rooms using a portable infrared spectrophotometry. Venous blood samples will be drawn from all residents. Oxidative stress will be evaluated by lymphocyte oxidative DNA damage (alkaline comet assay and chromatography), plasma lipid peroxidation (malondyaldehyde and 4-hydroxynonenal evaluated by chromatography and immunoassay, respectively), carbonyl proteins (immunoassay), and by the total antioxidant performance (TAP), determined by fluorescence. The immune system will be assessed by several plasma inflammatory and T helper cytokines by flow cytometry. It will be the first time that information about waste anesthetic gases with possible effects in the oxidative stress and immunological system in medical residents newly exposed will be assessed. There is no limit threshold in Brazil regarding to occupational exposure to anesthetics. This knowledge will certainly contribute to know these values and if they are in high levels. If so, we will evaluate the impact of this exposure in the recently exposed professionals, with direct impact on public health. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CHAOUL, MAURICIO MARTINS; BRAZ, JOSE REINALDO C.; LUCIO, LORENA MENDES C.; GOLIM, MARJORIE A.; BRAZ, LEANDRO GOBBO; BRAZ, MARIANA GOBBO. Does occupational exposure to anesthetic gases lead to increase of pro-inflammatory cytokines?. Inflammation Research, v. 64, n. 12, p. 939-942, DEC 2015. (13/13115-0, 13/21130-0)

BRAZ, MARIANA G.; CARVALHO, LORENA I. M.; CHEN, CHUNG-YEN O.; BLUMBERG, JEFFREY B.; SOUZA, KATINA M.; ARRUDA, NAYARA M.; FILHO, DANIEL A. A.; RESENDE, LUDIMILA O.; FARIA, RENATA T. B. G.; CANARIO, CLARA D'A; et al. High concentrations of waste anesthetic gases induce genetic damage and inflammation in physicians exposed for three years: A cross-sectional study. INDOOR AIR, v. 30, n. 3, p. 512-520, MAY 2020. (13/12120-0, 13/21130-0, 15/06420-7, 13/18075-7, 14/08661-9)

BRAZ, MARIANA G.; SOUZA, KATINA M.; LUCIO, LORENA M. C.; DI RENZO, GIULIA C. C.; FELICIANO, LUCIANA M.; MARCONDES, JOAO PAULO C.; OLIVER CHEN, C. -Y.; BRAZ, JOSE REINALDO C.; BRAZ, LEANDRO G.. Detrimental effects detected in exfoliated buccal cells from anesthesiology medical residents occupationally exposed to inhalation anesthetics: An observational study. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, v. 832, p. 61-64, AUG 2018. (13/21130-0, 13/05084-8)

BRAZ, MARIANA G.; SILVA, MARIANE A. P.; FIGUEIREDO, DRIELLE B. S.; AUN, ALINE G.; MARQUES, LAIS S. K.; LARA, JULIANA R.; BRAZ, LEANDRO G.. Genetic instability assessed by telomere length and micronucleus in physicians exposed to anesthetics. Environmental and Molecular Mutagenesis, JUN 2020. (13/21130-0, 13/05084-8, 16/15559-1)

BRAZ, MARIANA G.; SILVA, MARIANE A. P.; FIGUEIREDO, DRIELLE B. S.; AUN, ALINE G.; MARQUES, LAIS S. K.; LARA, JULIANA R.; BRAZ, LEANDRO G.. Genetic instability assessed by telomere length and micronucleus in physicians exposed to anesthetics. Environmental and Molecular Mutagenesis, v. 61, n. 8, p. 5-pg., 2020-06-24. (16/15559-1, 13/21130-0, 13/05084-8)

LUCIO, LORENA M. C.; BRAZ, MARIANA G.; DO NASCIMENTO JUNIOR, PAULO; BRAZ, JOSE REINALDO C.; BRAZ, LEANDRO G.. Riscos ocupacionais, danos no material genético e estresse oxidativo frente à exposição aos resíduos de gases anestésicos. Revista Brasileira de Anestesiologia, v. 68, n. 1, p. 33-41, JAN-FEB 2018. (13/21130-0)

LEANDRO GOBBO BRAZ; JOSÉ REINALDO CERQUEIRA BRAZ; GUILHERME APARECIDO SILVA CAVALCANTE; KÁTINA MENEGHETTI SOUZA; LORENA MENDES DE CARVALHO LUCIO; MARIANA GOBBO BRAZ. Comparação de resíduos de gases anestésicos em salas de operação com ou sem sistema de exaustão em hospital universitário brasileiro. Revista Brasileira de Anestesiologia, v. 67, n. 5, p. 516-520, 2017-10-00. (13/05084-8, 13/21130-0)

SILVA, MARIANE A. P.; FIGUEIREDO, DRIELLE B. S.; LARA, JULIANA R.; PASCHOALINOTTE, ELOISA E.; BRAZ, LEANDRO G.; BRAZ, MARIANA G.. Evaluation of genetic instability, oxidative stress, and metabolism-related gene polymorphisms in workers exposed to waste anesthetic gases. Environmental Science and Pollution Research, v. N/A, p. 15-pg., 2022-09-03. (13/21130-0, 18/20900-0, 13/05084-8)