The role of AMH signaling on medaka sex differentiation and gametogenesis

• Grant number: 14/50602-0
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2014
• End date: November 30, 2016
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Agreement: BAYLAT/StMBW - Bavarian Academic Center for Latin America and Bavarian State Ministry of Science and the Arts
• Mobility Program: SPRINT - Projetos de pesquisa - Mobilidade
• Principal Investigator: Rafael Henrique Nóbrega
• Grantee: Rafael Henrique Nóbrega
• Principal researcher abroad: Manfred Scharlt
• Institution abroad: Universität Würzburg, Germany
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 12/00423-6 - Paracrine and molecular regulation of the spermatogonial stem cell niche in zebrafish (Danio rerio) and the use of common carp (Cyprinus carpio) as a model to delay spermatogonial differentiation during puberty, AP.R

Abstract

Amh signaling is an important effector in the decision whether the bipotencial gonad anlage will become a testis or ovary. Studies with medaka hotei, where Amh type II receptor (Amhr2) is mutated showed an overproliferation of germ cells and a male-to-female sex reversal. Our hypothesis is that Amh regulates germ cell number, which may nfluence the sex differentiation decision in medaka. Moreover, Amh might have a role in adult gametogenesis. Therefore, we aimed to evaluate Amh signaling during sex differentiation and gametogenesis. For that transgenic lines overexpressing amhr2 or lacking amh/amhr2 will be generated in medaka. Genetic screening for Y chromosome contrasted with gonad histology will be performed to evaluate sex reversal. Moreover, number of germ cells, proliferation and steroid release will be conducted in each transgenic line to unravel the role of Amh signaling in medaka gametogenesis. All these data will provide evidences to better understand the role of Amh as an important effector in the control of germ cell number, which might influences the sex differentiation decision and gametogenesis in medaka. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DU, KANG; WUERTZ, SVEN; ADOLFI, MATEUS; KNEITZ, SUSANNE; STOECK, MATTHIAS; OLIVEIRA, MARCOS; NOBREGA, RAFAEL; ORMANNS, JENNY; KLOAS, WERNER; FERON, ROMAIN; et al. The genome of the arapaima (Arapaima gigas) provides insights into gigantism, fast growth and chromosomal sex determination. SCIENTIFIC REPORTS, v. 9, MAR 28 2019. (14/25313-4, 14/50602-0, 15/15631-1, 14/07620-7)