In vivo and in vitro effect of the antiinflammatory and antioxidant on dystrophic muscle fibers of Mdx mice

• Grant number: 13/17732-4
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2015
• End date: January 31, 2017
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Elaine Minatel
• Grantee: Elaine Minatel
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Maria Julia Marques

Abstract

The objective of this study is to analyze the effects "in vivo and in vitro" the association of inflammatory Diacerhein with antioxidant Vitamin E on the muscle fibers of dystrophic mdx mice. In the studies "in vivo", mdx mice with 14 days of postnatal life will be divided into 4 groups: (1) treated with saline, (2) treated with the combination of Diacerhein and Vitamin E, (3) treated with Diacerhein and (4) treated with Vitamin E. C57BL/10 mice will be used as control and will not receive any treatment. The diaphragm muscle of all experimental groups will be withdrawn and used for morphological and biochemical. Blood samples will also be removed and subjected to biochemical analysis. Studies "in vitro" will be performed with primary cultures of muscle cells of C57BL/10 and mdx mice. Muscle cells are treated or not with Diacerhein and Vitamin E and subjected to biochemical analysis. The results will be statistically analyzed. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MANCIO, RAFAEL DIAS; HERMES, TULIO DE ALMEIDA; MACEDO, ALINE BARBOSA; MIZOBUTI, DANIELA SAYURI; VALDUGA, AMANDA HARDUIM; RUPCIC, IAN FELLER; MINATEL, ELAINE. Vitamin E treatment decreases muscle injury in mdx mice. NUTRITION, v. 43-44, p. 39-46, NOV-DEC 2017. (14/01970-6, 13/17732-4)

MANCIO, R. D.; HERMES, T. A.; RUPCIC, I. F.; MINATEL, E.. Diacerhein effects on inflammatory and oxidative stress pathways in the diaphragm muscle of mdx mice.. MOLECULAR BIOLOGY OF THE CELL, v. 27, p. 1-pg., 2016-01-01. (14/25010-1, 13/17732-4, 14/01970-6)

MANCIO, RAFAEL DIAS; HERMES, TULIO DE ALMEIDA; MACEDO, ALINE BARBOSA; MIZOBUTI, DANIELA SAYURI; RUPCIC, IAN FELLER; MINATEL, ELAINE. Dystrophic phenotype improvement in the diaphragm muscle of mdx mice by diacerhein. PLoS One, v. 12, n. 8, AUG 7 2017. (14/01970-6, 13/17732-4)