Role of chemical signaling and auxiliary mechanisms in Salmonella enterica serovar Typhimurium virulence and other enteropathogens

• Grant number: 14/06779-2
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: March 01, 2015
• End date: April 30, 2019
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Cristiano Gallina Moreira
• Grantee: Cristiano Gallina Moreira
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 18/22042-0 - Chemical signaling and virulence in Escherichia coli enterohemorrhagic mediated by members of the microbiota, BP.MS; 16/12744-2 - Transcriptional YgiV role and VisP protein-protein interaction on chemical signaling and LPS assembly in Salmonella enterica sorovar Typhimurium pathogenesis, BP.DR; 17/19243-1 - Characterization of pathogenicity and chemical signaling of prototype strains and clinical samples of uropathogenic Escherichia coli against compound LED209, BP.MS; 15/26132-6 - Chemical signaling via QseC and QseE in Citrobacter rodentium, BP.IC

Abstract

Bacterial chemical signaling is a mechanism employed by several bacteria species to interact within surrounding microbiota and their host. Upon this interaction the pathogenic bacteria regulate their virulence traits. The 2-component system QseBC was described on chemical signaling of the Autoinducer-3, epinephrine, and norepinephrine in Salmonella enterica serovar Typhimurium, and a novel branch of pathogenic cascade regulation was raveled. Amongst these mechanisms a novel protein was described, VisP (Virulence and stress-related Periplasmic protein) (29). Its initial role, the interaction with LpxO enzyme (Dioxygenase Fe2+/ ±-ketoglutarate-dependent) and peptoglycan layer was previously shown (29). Further studies are necessary for a complete clarification of these mechanisms, specially related to TTSS encoded both by SPI-1 and SPI-2, and the role of O-antigen from the LPS of S. Typhimurium. Novel mechanisms briefly unveiled by transcriptomic analyses of QseC sensor kinase still need to be assessed. These mechanisms will be further investigated on this project to characterize these mechanisms in human pathogenic bacteria, evaluate their importance, understand novel processes, and validate these results in other members of the Enterobacteriaceae family. The full comprehension of the complex relationship between pathogenic bacteria, the microbiota, and the host is essential subject in microbial pathogenesis. The human intestine albeit a large variety of bacterial species, the majority are commensal bacteria, which suffer the pathogenic bacteria strike from time to time. The chemical signaling among bacteria is the central aspect of this association. The complete elucidation of these signals will be essential to understand their relationship, develop novel technologies and therapies in benefit to human life. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, PATRICK; LUSTRI, BRUNA C.; CASTILHO, IVANA GIOVANNETTI; FERREIRA, ADRIANO MARTISON; HERNANDES, RODRIGO T.; SCHEMBRI, MARK A.; MOREIRA, CRISTIANO G.. Genome profiling of fluoroquinolone-resistant uropathogenic Escherichia coli isolates from Brazil. Brazilian Journal of Microbiology, JUN 2021. (14/06779-2, 19/03049-7)

LUSTRI, BRUNA C.; SPERANDIO, VANESSA; MOREIRA, CRISTIANO G.. Bacterial Chat: Intestinal Metabolites and Signals in Host-Microbiota-Pathogen Interactions. Infection and Immunity, v. 85, n. 12, DEC 2017. (14/06779-2)

DA SILVA, PATRICK; LUSTRI, BRUNA C.; CASTILHO, IVANA GIOVANNETTI; FERREIRA, ADRIANO MARTISON; HERNANDES, RODRIGO T.; SCHEMBRI, MARK A.; MOREIRA, CRISTIANO G.. Genome profiling of fluoroquinolone-resistant uropathogenic Escherichia coli isolates from Brazil. Brazilian Journal of Microbiology, v. 52, n. 3, p. 1067-1075, SEP 2021. (14/06779-2, 19/03049-7)

SIMEI AQUARONI, NAYARA AP.; NAKAHATA, DOUGLAS H.; LAZARINI, SILMARA C.; RESENDE, FLAVIA A.; CANDIDO, AMANDA L. P.; BARUD, HERNANE DA SILVA; CLARO, AMANDA MARIA; DE CARVALHO, JOAO ERNESTO; RIBEIRO, CAMILA M.; PAVAN, FERNANDO R.; et al. Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support. Journal of Inorganic Biochemistry, v. 212, NOV 2020. (17/19243-1, 15/09833-0, 17/16278-9, 18/12062-4, 16/07729-4, 13/07276-1, 18/12590-0, 18/25512-8, 14/06779-2, 19/03049-7, 18/02344-2, 18/00163-0, 15/20882-3)

MACHADO RIBEIRO, TAMARA RENATA; LUSTRI, BRUNA CARDINALI; ELIAS, WALDIR P.; MOREIRA, CRISTIANO GALLINA. QseC Signaling in the Outbreak O104:H4 Escherichia coli Strain Combines Multiple Factors during Infection. Journal of Bacteriology, v. 201, n. 17, SEP 2019. (14/06779-2)

MELCHIOR, KARINE; MOREIRA, CRISTIANO GALLINA. Novel non-flagellated surface motility mediated by chemical signaling in Citrobacter rodentium. Brazilian Journal of Microbiology, v. 50, n. 4, p. 881-886, OCT 2019. (14/06779-2, 18/22042-0)

BRAZ, VANIA SANTOS; MELCHIOR, KARINE; MOREIRA, CRISTIANO GALLINA. Escherichia coli as a Multifaceted Pathogenic and Versatile Bacterium. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 10, DEC 21 2020. (14/06779-2, 19/03049-7, 18/22412-2, 18/22042-0)