Characterization of pathogenicity and chemical signaling of prototype strains and clinical samples of uropathogenic Escherichia coli against compound LED209

• Grant number: 17/19243-1
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): April 01, 2018
• Effective date (End): July 31, 2019
• Field of knowledge: Biological Sciences - Microbiology
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Cristiano Gallina Moreira
• Grantee: Bruna Cardinali Lustri
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated research grant: 14/06779-2 - Role of chemical signaling and auxiliary mechanisms in Salmonella enterica serovar Typhimurium virulence and other enteropathogens, AP.JP

Abstract

Urinary tract infections (UTIs) are common worldwide, with Uropathogenic Escherichia coli (UPEC) being the pathogen responsible for most infections, leading to the development of cystitis and acute pyelonephritis, in addition to being responsible for 35-45 % of cases of Acute Renal Failure (ARF). The classical virulence factors of UPEC are linked to the expression of fimbriae P, ±-hemolysin, aerobactin, resistance to serum and encapsulation. The chemical signaling facilitating the colonization process and establishment of the pathogenesis through factors with mediate inter- and intra-kingdom communication. The two-component QseBC system is able to recognize signals produced by the host, such as adrenergic hormones epinephrine (Epi) and norepinephrine (NE), as well as signaling molecules produced by other bacteria, such as the auto-inducer-3 (AI-3). which may regulate and activate the expression of pathogen virulence genes. The LED209 is a molecule that was developed with the purpose of blocking such a signaling pathway, inhibiting the transcription of virulence factors, not interfering with bacterial growth, but making it difficult to colonize and establish the disease. The aim of the present project is investigate in vitro and in vivo how the chemical signaling via QseBC assists in the pathogenesis and virulence of prototype strains EC958, UTI89 and CFT073, as well as recent clinical isolates from the State of São Paulo of UPEC strains, also employing the compound LED209 for blocking this pathway. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SIMEI AQUARONI, NAYARA AP.; NAKAHATA, DOUGLAS H.; LAZARINI, SILMARA C.; RESENDE, FLAVIA A.; CANDIDO, AMANDA L. P.; BARUD, HERNANE DA SILVA; CLARO, AMANDA MARIA; DE CARVALHO, JOAO ERNESTO; RIBEIRO, CAMILA M.; PAVAN, FERNANDO R.; et al. Antibacterial activities and antiproliferative assays over a tumor cells panel of a silver complex with 4-aminobenzoic acid: Studies in vitro of sustained release using bacterial cellulose membranes as support. Journal of Inorganic Biochemistry, v. 212, NOV 2020. (17/19243-1, 15/09833-0, 17/16278-9, 18/12062-4, 16/07729-4, 13/07276-1, 18/12590-0, 18/25512-8, 14/06779-2, 19/03049-7, 18/02344-2, 18/00163-0, 15/20882-3)

LUSTRI, WILTON R.; LAZARINI, SILMARA C.; AQUARONI, NAYARA AP SIMEI; RESENDE, FLAVIA A.; ALEIXO, NADIA A.; PEREIRA, DOUGLAS H.; LUSTRI, BRUNA CARDINALI; MOREIRA, CRISTIANO GALLINA; RIBEIRO, CAMILA M.; PAVAN, FERNANDO R.; et al. A new complex of silver(I) with probenecid: Synthesis, characterization, and studies of antibacterial and extended spectrum beta-lactamases (ESBL) inhibition activities. Journal of Inorganic Biochemistry, v. 243, p. 12-pg., 2023-03-30. (18/00163-0, 19/03049-7, 15/09833-0, 15/20882-3, 18/12062-4, 17/16278-9, 21/07458-9, 17/19243-1, 20/13497-4, 18/12590-0, 19/26696-8, 18/00187-7, 21/08717-8, 18/02344-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

LUSTRI, Bruna Cardinali. Characterization of pathogenicity and chemical signaling of prototype strains and clinical samples of uropathogenic Escherichia coli against LED209. 2019. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara Araraquara.