Antioxidant activity of green propolis in model system

• Grant number: 14/18227-4
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2015
• End date: March 31, 2017
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Thais Maria Ferreira de Souza Vieira
• Grantee: Thais Maria Ferreira de Souza Vieira
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated researchers: Severino Matias de Alencar
• Associated research grant(s): 15/50437-1 - Optimizing antioxidant extraction and milk processing conditions for reduced lipid oxidation, AP.R SPRINT

Abstract

The lipid oxidation is considered the second cause most important for food deterioration. With the aim to retard or minimized it, the use of antioxidant compounds is necessary. Most antioxidants used today has been originated from synthetic sources, however they have restrictions for use in food because their toxic effects in human health. In consequence in the last decade there was an increase in researches on antioxidants from natural sources, with the aim to identify potentials supplies of these compounds. The aim of this research is to produce hydroalcoholic extracts of green propolis with high antioxidant activity and to evaluate their activity in lipid model systems. The extracts will be produced following the Response Surface Methodology, with the purpose to obtain extracts with maximum antioxidant activity in in vitro essays, using the ORAC (Oxygen-Radical Absorbance Capacity) methodologie. Phenolics compounds in the select extract will be identified and quantified by HPLC/MS. The evaluation of extracts in lipid model system (unsaturated vegetable oil and emulsion) will be performed in accelerated tests: oxidation started by FeCl3, and Oven test followed by spectrophotometric evaluation of hydroperoxides in samples. Volatiles compounds in these samples will be evaluated, using micro extraction in solid phase (SPME) by gas chromatography-mass spectrometry (GS/MS). The antioxidant activity of green propolis extracts will be compared to the effectiveness of synthetics antioxidants permitted by Brazilian legislation as TBHQ, BHA and BHT. Products (oil and/or emulsion) added of propolis extracts which presented the better oxidative stability will be submitted to sensorial analysis in order verify the effect on odour and colour. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

AUGUSTO-OBARA, THALITA RIQUELME; DE OLIVEIRA, JACQUELINE; DA GLORIA, EDUARDO MICOTTI; FILLET SPOTO, MARTA HELENA; GODOY, KARINA; FERREIRA DE SOUZA VIEIRA, THAIS MARIA; SCHEUERMANN, ERICK. Benefits of superfine grinding method on antioxidant and antifungal characteristic of Brazilian green propolis extract. Scientia Agricola, v. 76, n. 5, p. 398-404, SEP-OCT 2019. (14/18227-4, 14/50235-7)

DIAS, FERNANDA FURLAN GONCALVES; AUGUSTO-OBARA, THALITA RIQUELME; HENNEBELLE, MARIE; CHANTIENG, SIRIYAKORN; OZTURK, GULUSTAN; TAHA, AMEER Y.; DE SOUZA VIEIRA, THAIS MARIA FERREIRA; BELL, JULIANA MARIA LEITE NOBREGA DE MOURA. Effects of industrial heat treatments on bovine milk oxylipins and conventional markers of lipid oxidation. PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, v. 152, JAN 2020. (14/18227-4, 15/50437-1)

CAVALARO, RENATA IARA; DA CRUZA, RICHTIER GONCALVES; DUPONT, SEBASTIEN; BELL, JULIANA MARIA LEITE NOBREGA DE MOURA; VIEIRA, THAIS MARIA FERREIRA DE SOUZA. In vitro and in vivo antioxidant properties of bioactive compounds from green propolis obtained by ultrasound-assisted extraction. FOOD CHEMISTRY-X, v. 4, DEC 30 2019. (14/18227-4, 15/50437-1)

CAVALARO, RENATA IARA; DE FREITAS FABRICIO, LUIS FELIPE; FERREIRA DE SOUZA VIEIRA, THAIS MARIA. Ultrasound-Assisted Extraction of Antioxidants from Baccharis dracunculifolia and Green Propolis. PROCESSES, v. 8, n. 12, DEC 2020. (15/50437-1, 14/18227-4)

AUGUSTO-OBARA, THALITA RIQUELME; DE OLIVEIRA, JACQUELINE; DA GLORIA, EDUARDO MICOTTI; FILLET SPOTO, MARTA HELENA; GODOY, KARINA; FERREIRA DE SOUZA VIEIRA, THAIS MARIA; SCHEUERMANN, ERICK. Benefits of superfine grinding method on antioxidant and antifungal characteristic of Brazilian green propolis extract. CIENTIA AGRICOL, v. 76, n. 5, p. 7-pg., 2019-09-01. (14/18227-4, 14/50235-7)