Abstract
Multi-User Equipment approved in grant 2014/17526-8: Electron Paramagnetic Resonance (EPR) spectrometer - X Band is the most straightforward-to-use technique for studying free radicals and other paramagnetic species and has therefore established itself in biomedical research and for clinical applications. In additional, this technique also have been extensively employed in many areas in physics, chemistry and biology. The basic concepts of EPR are analogous to those of nuclear magnetic resonance (NMR), but it is electron spins that are excited instead of the spins of atomic nuclei. EPR spectroscopy is particularly useful for studying metal complexes or organic radicals. EPR found its applications for investigations on the structures of this protein aggregates, but also of other proteins or protein complexes with medical relevance the technique of site-directed spin labeling (SDSL) - the site- specific introduction of paramagnetic reporter groups into proteins or nucleic acids - is nowadays frequently used, especially in cases where other structural techniques are not. Moreover, by direct detection of free radicals - also in combination with spin trapping methods, or application of SDSL, EPR spectroscopy can be also used for diagnostic purposes, e.g. in cancer diagnostics. (AU)
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