Economic impact and profile of etiologic agents of bovine mastitis

• Grant number: 14/17411-6
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2015
• End date: February 28, 2018
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Animal Production
• Principal Investigator: Marcos Veiga dos Santos
• Grantee: Marcos Veiga dos Santos
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The general objectives of this proposal are to evaluate: a) etiologic profile and antimicrobial susceptibility of pathogens causing clinical mastitis and to describe the qualitative and quantitative aspects relating to the use of antimicrobials; b) efficiency of on farm culture program, specially on the use of antimicrobials and economic aspects related to expenses incurred for clinical mastitis therapy; production losses and milk composition changes associated with somatic cell count at herd level. The specific objectives of this proposal will be achieved by performing of two subprojects (1 e 2) and the following objectives: Subproject 1: Impact of subclinical mastitis on production cost and milk quality of dairy herds. The general objective of this subproject is to evaluate the effects of subclinical intramammary infections caused by primary pathogens on somatic cell count and milk yield and composition of dairy cows at mammary quarter level; In addition, the Subproject 1 aims to evaluate production losses and changes in milk composition at herd level by SCC; and to determine the economic impact of subclinical mastitis caused by major pathogens in dairy herds. Three experiments will be performed to achieve the objectives of the Subproject 1: Experiment 1 - Effect of subclinical mastitis caused by primary pathogens on production and milk composition of dairy cows at mammary quarter level; Experiment 2 - Production losses and milk composition changes caused by subclinical mastitis at herd level; Experiment 3 - economic impact of subclinical mastitis caused by primary pathogens on the milk production cost of dairy herd. Subproject 2: Etiological profile of clinical mastitis and antimicrobial use in dairy herds. General objective: to determine the etiologic profile and antimicrobial susceptibility of clinical mastitis pathogens, and to describe the qualitative and quantitative aspects associated with antimicrobial drug usage for treatment of mastitis in dairy herds. Additionally, the study aims to evaluate the effectiveness of the on farm microbiological culture (OFC) on use of antimicrobials and economic evaluation of clinical mastitis therapy protocols. Three experiments will be performed to achieve the objectives of Subproject 2: Experiment 1 - Determination of etiologic profile of clinical mastitis in dairy herds; Experiment 2 - Quantification of antimicrobial drug usage for treatment of mastitis; and Experiment 3 - Economic approach and evaluation of risk factors related to a program of on farm microbiological culture. Overall, this proposal is focused on the etiologic and epidemiologic evaluation of intramammary infections, aiming to promote alternatives to mastitis control, reduction of somatic cell count and reduction of antimicrobials used for clinical mastitis therapy. (AU)

Scientific publications

(15)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GONCALVES, JULIANO LEONEL; LYMAN, ROBERTA L.; HOCKETT, MITCHELL; RODRIGUEZ, RUDY; DOS SANTOS, MARCOS VEIGA; ANDERSON, KEVIN L.. Using milk leukocyte differentials for diagnosis of subclinical bovine mastitis. JOURNAL OF DAIRY RESEARCH, v. 84, n. 3, p. 309-317, AUG 2017. (13/07914-8, 14/17411-6, 13/23613-8, 15/04570-1)

TOMAZI, TIAGO; FERREIRA, GABRIEL C.; ORSI, ALESSANDRA M.; GONCALVES, JULIANO L.; OSPINA, PAULA A.; NYDAM, DARYL V.; MORONI, PAOLO; DOS SANTOS, MARCOS V.. Association of herd-level risk factors and incidence rate of clinical mastitis in 20 Brazilian dairy herds. PREVENTIVE VETERINARY MEDICINE, v. 161, p. 9-18, DEC 1 2018. (14/17411-6)

TOMAZI, T.; COURA, F. M.; GONCALVES, J. L.; HEINEMANN, M. B.; SANTOS, M. V.. Antimicrobial susceptibility patterns of Escherichia coli phylogenetic groups isolated from bovine clinical mastitis. JOURNAL OF DAIRY SCIENCE, v. 101, n. 10, p. 9406-9418, OCT 2018. (13/22286-3, 14/17411-6, 15/10332-6)

GONCALVES, JULIANO L.; CUE, I, ROGER; LIMA NETTO, EXPEDITO P.; GAMEIRO, AUGUSTO H.; DOS SANTOS, V, MARCOS. Herd-level associations between somatic cell counts and economic performance indicators in Brazilian dairy herds. JOURNAL OF DAIRY SCIENCE, v. 104, n. 2, p. 1855-1863, FEB 2021. (14/17411-6, 15/04570-1, 13/23613-8)

TOMAZI, TIAGO; FREU, GUSTAVO; ALVES, BRUNA GOMES; DE SOUZA FILHO, ANTONIO FRANCISCO; HEINEMANN, MARCOS BRYAN; DOS SANTOS, MARCOS VEIGA. Genotyping and antimicrobial resistance of Streptococcus uberis isolated from bovine clinical mastitis. PLoS One, v. 14, n. 10, OCT 22 2019. (15/10332-6, 13/22286-3, 14/17411-6)

FREU, GUSTAVO; TOMAZI, TIAGO; FILHO, ANTONIO F. S.; HEINEMANN, MARCOS B.; DOS SANTOS, MARCOS, V. Antimicrobial Resistance and Molecular Characterization of Staphylococcus aureus Recovered from Cows with Clinical Mastitis in Dairy Herds from Southeastern Brazil. ANTIBIOTICS-BASEL, v. 11, n. 4, p. 11-pg., 2022-04-01. (13/22286-3, 14/17411-6, 15/10332-6)

GONCALVES, JULIANO L.; CUE, ROGER I.; BOTARO, BRUNO G.; HORST, JOSE A.; VALLOTO, ALTAIR A.; SANTOS, MARCOS V.. Milk losses associated with somatic cell counts by parity and stage of lactation. JOURNAL OF DAIRY SCIENCE, v. 101, n. 5, p. 4357-4366, MAY 2018. (14/17411-6, 13/23613-8, 15/04570-1)

MARTINS, LARISSA; GONCALVES, JULIANO L.; LEITE, RENATA F.; TOMAZI, TIAGO; RALL, VERA L. M.; SANTOS, V, MARCOS. Association between antimicrobial use and antimicrobial resistance of Streptococcus uberis causing clinical mastitis. JOURNAL OF DAIRY SCIENCE, v. 104, n. 11, p. 12030-12041, NOV 2021. (13/23613-8, 18/23040-1, 14/17411-6, 13/22286-3)

GONCALVES, JULIANO LEONEL; LEE, SARAH H. I.; CAMARGO, CARLOS H.; ZANELLA, ROSEMEIRE COBO; SILVA, NATHALIA C. C.; RALL, VERA. L. M.; CUE, ROGER I.; DOS SANTOS, MARCOS V.. Molecular characterization of persistent subclinical mastitis-causing Staphylococcus aureus from dairy farms. Brazilian Journal of Microbiology, v. 54, n. 2, p. 9-pg., 2023-03-21. (15/04570-1, 14/17411-6, 13/23613-8)

GONCALVES, J. L.; KAMPHUIS, C.; MARTINS, C. M. M. R.; BARREIRO, J. R.; TOMAZI, T.; GAMEIRO, A. H.; HOGEVEEN, H.; DOS SANTOS, V, M.. Bovine subclinical mastitis reduces milk yield and economic return. LIVESTOCK SCIENCE, v. 210, p. 25-32, APR 2018. (14/17411-6, 15/04570-1, 13/23613-8, 13/07914-8)

GONCALVES, J. L.; KAMPHUIS, C.; VERNOOIJ, H.; ARAUJO, JR., J. P.; GRENFELL, R. C.; JULIANO, L.; ANDERSON, K. L.; HOGEVEEN, H.; DOS SANTOS, V, M.. Pathogen effects on milk yield and composition in chronic subclinical mastitis in dairy cows. VETERINARY JOURNAL, v. 262, AUG 2020. (15/04570-1, 13/23613-8, 13/07914-8, 14/17411-6)

MARTINS, LARISSA; BARCELOS, MELINA MELO; CUE, ROGER I.; ANDERSON, KEVIN L.; SANTOS, MARCOS VEIGA DOS; GONCALVES, JULIANO LEONEL. Chronic subclinical mastitis reduces milk and components yield at the cow level. JOURNAL OF DAIRY RESEARCH, v. 87, n. 3, p. 298-305, AUG 2020. (14/17411-6, 13/23613-8, 15/04570-1, 15/00142-5)

BARCELOS, M. M.; MARTINS, L.; GRENFELL, R. C.; JULIANO, L.; ANDERSON, K. L.; DOS SANTOS, M. V.; GONCALVES, J. L.. Comparison of standard and on-plate extraction protocols for identification of mastitis-causing bacteria by MALDI-TOF MS. Brazilian Journal of Microbiology, v. 50, n. 3, p. 849-857, JUL 2019. (13/23613-8, 14/22076-1, 14/17411-6)

TOMAZI, TIAGO; DOS SANTOS, MARCOS VEIGA. Antimicrobial use for treatment of clinical mastitis in dairy herds from Brazil and its association with herd-level descriptors. PREVENTIVE VETERINARY MEDICINE, v. 176, MAR 2020. (14/17411-6)

TOMAZI, TIAGO; DE SOUZA FILHO, ANTONIO FRANCISCO; HEINEMANN, MARCOS BRYAN; DOS SANTOS, MARCOS VEIGA. Molecular characterization and antimicrobial susceptibility pattern of Streptococcus agalactiae isolated from clinical mastitis in dairy cattle. PLoS One, v. 13, n. 6, JUN 21 2018. (13/22286-3, 14/17411-6, 15/10332-6)