Magnetic bionanocatalysts: preparation, characterization, properties and use in protein and peptide chemistry

• Grant number: 15/14360-4
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2015
• End date: March 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Maria Teresa Machini
• Grantee: Maria Teresa Machini
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Liane Marcia Rossi

Abstract

This application aims to get financial support to continue some of our ongoing studies which were supported by FAPESP up to last January. The studies have in common the goal of establishing new clean technologies for Organic Synthesis (Fine Chemistry) and Biochemistry (Chemistry of Peptides and Proteins) based on the combination of biocatalysis and nanotechnology. The studies proposed will be done in the following sequence: (i) study on the preparation and characterization of enzymatic magnetic nanocatalysts with synthetic and hydrolytic activities (Fe3O4@silica-thermolysin and -bovine trypsin), (ii) examination of the properties of such new Fe3O4@silica-enzymes, (iii) study on the combined use of Fe3O4@silica-enzymes and the palladium magnetic nanocatalysts Fe3O4@silica-Pd as catalysts for the synthesis of dipeptides of high scientific and commercial values, (iii) study on the feasibility of applying the Fe3O4@silica-enzymes obtained for proteolysis/sequencing/identification of proteins. Therefore, methods, techniques, procedures and protocols often used in Organic Synthesis, Peptide and Protein Chemistries, Enzymology, Proteomics and Chemistry of Materials will be employed. Due to the interdisciplinary nature of the studies, we will collaborate with experts in Catalysis and Nanomaterials, Enzymology and Organic Synthesis. Graduate and undergraduate students under my supervision will be involved. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GARCIA, PAMELA SOTO; BRUM, DORALINA GUIMARAES; OLIVEIRA JR, OSVALDO N.; HIGA, AKEMI MARTINS; MAGALHAES IERICH, JESSICA CRISTIANE; MORAES, ARIANA DE SOUZA; SHIMIZU, FLAVIO MAKOTO; OKUDA-SHINAGAWA, NANCY M.; PERONI, LUIS ANTONIO; DA GAMA, PAULO DINIZ; et al. Nanoimmunosensor based on atomic force spectroscopy to detect anti-myelin basic protein related to early-stage multiple sclerosis. ULTRAMICROSCOPY, v. 211, APR 2020. (14/50869-6, 12/50839-4, 13/14262-7, 14/12082-4, 14/21530-0, 15/06847-0, 15/14360-4)

WEGERMANN, CAMILA ANCHAU; PIROTA, VALENTINA; MONZANI, ENRICO; CASELLA, LUIGI; COSTA, LUIZ ANTONIO SODRE; NOVATO, WILLIAN TASSIO GOMES; MACHINI, M. TERESA; FERREIRA, ANA MARIA DA COSTA. Interaction studies of oxindole-derivatives with beta-amyloid peptides inhibiting its aggregation induced by metal ions. Journal of Inorganic Biochemistry, v. 245, p. 16-pg., 2023-05-06. (11/50318-1, 15/14360-4, 13/07937-8)

MARCELINO, LARISSA; PUPPIN-RONTANI, JULIA; COUTTE, FRANCOIS; TERESA MACHINI, M.; ETCHEGARAY, AUGUSTO; PUPPIN-RONTANI, REGINA MARIA. Surfactin application for a short period (10/20 s) increases the surface wettability of sound dentin. Amino Acids, v. 51, n. 8, p. 1233-1240, AUG 2019. (15/14360-4)

LIMA, THAINA A.; ETCHEGARAY, AUGUSTO; MACHINI, M. TERESA. Design, synthesis and valued properties of surfactin oversimplified analogues. Amino Acids, v. 52, n. 1, p. 25-33, JAN 2020. (13/20570-6, 14/06784-6, 15/14360-4)

CARVALHO, RODRIGO R. V.; PERES, TANARA V.; LIRIA, CLEBER W.; MACHINI, M. TERESA; ASCHNER, MICHAEL; ESPOSITO, BRENO P.. Conjugates of desferrioxamine and aromatic amines improve markers of iron-dependent neurotoxicity. BIOMETALS, v. 34, n. 2, JAN 2021. (15/14360-4, 18/19684-0, 16/03709-9)

MORAES, ARIANA DE SOUZA; BRUM, DORALINA GUIMARAES; MAGALHAES IERICH, JESSICA CRISTIANE; HIGA, AKEMI MARTINS; JABUR ASSIS, AMANDA STEFANIE; MIYAZAKI, CELINA MASSUMI; SHIMIZU, FLAVIO MAKOTO; PERONI, LUIS ANTONIO; MACHINI, M. TERESA; BARREIRA, AMILTON ANTUNES; et al. A highly specific and sensitive nanoimmunosensor for the diagnosis of neuromyelitis optica spectrum disorders. SCIENTIFIC REPORTS, v. 9, NOV 6 2019. (16/19387-0, 14/50869-6, 13/14262-7, 14/15093-7, 12/50839-4, 14/12082-4, 15/05283-6, 15/06847-0, 15/14360-4)

PIZZOLATO-CEZAR, LUIS R.; OKUDA-SHINAGAWA, NANCY M.; TERESA MACHINI, M.. Combinatory Therapy Antimicrobial Peptide-Antibiotic to Minimize the Ongoing Rise of Resistance. FRONTIERS IN MICROBIOLOGY, v. 10, p. 5-pg., 2019-08-09. (15/14360-4)