TENDON-BONE JUNCITION: biomechanical, histological and molecular study in rabbits

• Grant number: 13/25046-3
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2015
• End date: May 31, 2018
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Olavo Pires de Camargo
• Grantee: Olavo Pires de Camargo
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Alexandre Leme Godoy dos Santos ; Cesar Augusto Martins Pereira ; César de César Neto ; Gustavo Bispo dos Santos ; Marcos Hideyo Sakaki ; Pedro Augusto Pontin ; Tulio Diniz Fernandes

Abstract

Tendon-bone junction healing is a complex process that is prone to various flaws. Clinically, during orthopedic medical practice , the need for this healing process to occur quickly and effectively is very important, especially in the treatment of the insertional tendon tears and in the employment of tendon transfer surgical techniques.The clinical management of these lesions is often paradoxical. Because of it's slow and gentle healing, it is necessary, in the early post-lesional or post-surgical phase, to keep some kind of immobilization and load protection of the affected area. These measures, however, lead to a delay in the return to normal activities of the patient, delaying its functional recovery.On account of these paradoxical characteristics, it is of great interest in the medical literature the development of behavioral and therapeutic methods to optimize and to shorten the healing period, however, avoid leading to fatigue and loosening of surgical repair , as well as delaying in the rehabilitation protocol.In this sense , looking up the possible variables responsible for interfering with the final quality and speed of integration of insertion osteotendínea .Taking these parameters as a starting point , our proposal asks the true role and influence of growth factors , in particular the family of bone morphogenetic proteins ( BMPs ) and their biological cascades in the healing process above . The project compares , through biomechanical analysis , histological and molecular experimental animal model in rabbits , the results obtained from the use of two surgical techniques traditionally used in orthopedic practice to rehabilitate a torn tendon insertional .General ObjectivesCompare the different phases of healing JOT Achilles tendon of rabbits , the results obtained through the use of two surgical techniques traditional tendon reinsertion ( suture through the bone tunnel and suture anchors ) . With this objective will be assessed : Standard in situ expression of BMPs track by means of the technique of immunohistochemistry. Levels of gene expression of these proteins by means of PCR in real time. Biomechanical strength in test with cyclic loads . (AU)