Biological process development of rich phenolics extracts from citrus waste industry and evaluation of bioactivity from extracts

• Grant number: 15/04555-2
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2016
• End date: December 31, 2018
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Gabriela Alves Macedo
• Grantee: Gabriela Alves Macedo
• Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Juliana Alves Macedo

Abstract

Phenolic compounds have been the focus of food science research for its action in human metabolism, acting in the prevention of some chronic diseases or, as important antioxidants in the food industry. Among the growing occurence of chronic diseases in our country, many are associated with obesity, and the high incidence of obesity has become a public health problem. Thus, various strategies and products have been evaluated in an attempt to prevent and treat excessive body fat and its complications. Among the compounds studied, we highlight the importance of phenolic present in food. However, conventional processes for obtaining these compounds are currently unattractive. These processes generate large amounts of waste, degrade the compounds of interest and reduce the yield. One possible alternative is the biological process, which can be enzymatic or fermentative. This study aims to obtain phenolic compounds by biotransformation using citrus residues . The biotransformation will perform fermentative and enzymatic processes and the conditions of incubation temperature, particle size of the residue, type and enzyme concentration as well as reaction time Will be explored. In the extracts obtained, the characterization of the phenolic profile by chromatography and evaluation of antioxidant potential in vitro DPPH and ORAC will be performed. The antiobesogênic biologic potential will be measured in cultured cell 3T3-L1 adipocytes, verifying the effect of the citrus biotransformed extract on the induction of lipolysis and lipogenesis, as potential indicative for further studies. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MENEZES BARBOSA, PAULA DE PAULA; RUVIARO, AMANDA ROGGIA; MARTINS, ISABELA MATEUS; MACEDO, JULIANA ALVES; LAPOINTE, GISELE; MACEDO, GABRIELA ALVES. nzyme-assisted extraction of flavanones from citrus pomace: Obtention of natural compounds with anti-virulence and anti-adhesive effect against Salmonella enterica subsp. enterica serovar Typhimuriu. FOOD CONTROL, v. 120, FEB 2021. (15/04555-2)

RUVIARO, AMANDA ROGGIA; MENEZES BARBOSA, PAULA DE PAULA; MACEDO, GABRIELA ALVES. Enzyme-assisted biotransformation increases hesperetin content in citrus juice by-products. Food Research International, v. 124, n. SI, p. 213-221, OCT 2019. (15/04555-2)

MENEZES BARBOSA, PAULA DE PAULA; RUVIARO, AMANDA ROGGIA; MARTINS, ISABELA MATEUS; MACEDO, JULIANA ALVES; LAPOINTE, GISELE; MACEDO, GABRIELA ALVES. Effect of enzymatic treatment of citrus by-products on bacterial growth, adhesion and cytokine production by Caco-2 cells. FOOD & FUNCTION, v. 11, n. 10, p. 8996-9009, OCT 1 2020. (15/04555-2)

MENEZES BARBOSA, PAULA DE PAULA; RUVIARO, AMANDA ROGGIA; MACEDO, GABRIELA ALVES. Comparison of different Brazilian citrus by-products as source of natural antioxidants. FOOD SCIENCE AND BIOTECHNOLOGY, v. 27, n. 5, p. 1301-1309, OCT 2018. (15/04555-2)

RUVIARO, AMANDA ROGGIA; MENEZES BARBOSA, PAULA DE PAULA; ALEXANDRE, EDUARDO COSTA; OLIVEIRA JUSTO, ALBERTO FERNANDO; ANTUNES, EDSON; MACEDO, GABRIELA ALVES. Aglycone-rich extracts from citrus by-products induced endothelium -independent relaxation in isolated arteries. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 23, JAN 2020. (15/04555-2)

BARBOSA, PAULA DE PAULA MENEZES; RUVIARO, AMANDA ROGGIA; MACEDO, GABRIELA ALVES. Conditions of enzyme-assisted extraction to increase the recovery of flavanone aglycones from pectin waste. JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, NOV 2020. (15/04555-2)

RUVIARO, AMANDA ROGGIA; MENEZES BARBOSA, PAULA DE PAULA; MARTINS, ISABELA MATEUS; ALVES DE AVILA, AMANDA REJANE; NAKAJIMA, VANIA MAYUMI; DOS PRAZERES, ALINE RODRIGUES; MACEDO, JULIANA ALVES; MACEDO, GABRIELA ALVES. Flavanones biotransformation of citrus by-products improves antioxidant and ACE inhibitory activities in vitro. FOOD BIOSCIENCE, v. 38, DEC 2020. (15/04555-2)

MENEZES BARBOSA, PAULA DE PAULA; RUVIARO, AMANDA ROGGIA; MACEDO, GABRIELA ALVES. Conditions of enzyme-assisted extraction to increase the recovery of flavanone aglycones from pectin waste. JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE, v. 58, n. 11, p. 4303-4312, NOV 2021. (15/04555-2)

NAKAJIMA, VANIA MAYUMI; MOALA, TAIS; CARIA, CINTIA RABELO E PAIVA; MOURA, CAROLINA SOARES; AMAYA-FARFAN, JAIME; GAMBERO, ALESSANDRA; MACEDO, GABRIELA ALVES; MACEDO, JULIANA ALVES. Biotransformed citrus extract as a source of anti-inflammatory polyphenols: Effects in macrophages and adipocytes. Food Research International, v. 97, p. 37-44, JUL 2017. (15/04555-2, 13/06494-5)

MENEZES BARBOSA, PAULA DE PAULA; RUVIARO, AMANDA ROGGIA; MARTINS, ISABELA MATEUS; MACEDO, JULIANA ALVES; LAPOINTE, GISELE; MACEDO, GABRIELA ALVES. Enzyme-assisted extraction of flavanones from citrus pomace: Obtention of natural compounds with anti-virulence and anti-adhesive effect against Salmonella enterica subsp. enterica serovar Typhimurium. FOOD CONTROL, v. 120, p. 11-pg., 2021-02-01. (15/04555-2)

RUVIARO, AMANDA ROGGIA; MENEZES BARBOSA, PAULA DE PAULA; MACEDO, GABRIELA ALVES. Enzyme-assisted biotransformation increases hesperetin content in citrus juice by-products. Food Research International, v. 124, p. 9-pg., 2019-10-01. (15/04555-2)