The immunomodulatory role of extracellular vesicles shedding from infected macrophages by the Trypanosoma cruzi.

• Grant number: 16/01917-3
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2016
• End date: August 31, 2018
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Ana Claudia Trocoli Torrecilhas
• Grantee: Ana Claudia Trocoli Torrecilhas
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

Infective forms of Trypanosoma cruzi, the causative agent of Chagas disease release vesicles enriched with glycoproteins that when injected into mice modulate parasite infection. The vesicles released by trypomastigotes of Y strain increases parasitism and inflammation of the heart tissue via the action of TNF-±, IL-12 and NO. Nogueira et al (2015) showed that vesicles isolated from YuYu, CL-14 and Y strains induced NO production, TNF-± and IL-6 via TLR2. Vesicles from Colombiana strain induced levels below the threshold of detection of these pro-inflammatory cytokines and NO. Vesicles of all analyzed strains activated the MAPK signaling pathway, but this activation was delayed in Colombian strain, explaining the low production of pro-inflammatory cytokines. Those results showed that vesicles released by trypomastigotes of different isolates caused cellular pro-inflammatory stimulation at various levels via the TLR2 receptor, which can be related to the progress of infection in each of the various strains. The objective of this study is to understand how vesicles released by macrophage infected with T. cruzi activate the immune response and inflammation. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VASCONCELOS, CAMILLA I.; VARELA, MARINA T.; TORRECILHAS, ANA C.; FERNANDES, JOAO P. S.. Pyrazinoates as antiparasitic agents against Trypanosoma cruzi. ARCHIV DER PHARMAZIE, v. 351, n. 11, NOV 2018. (16/01917-3, 18/03918-2, 16/25028-3)

SOARES, RODRIGO P.; XANDER, PATRICIA; COSTA, ADRIANA OLIVEIRA; MARCILLA, ANTONIO; MENEZES-NETO, ARMANDO; DEL PORTILLO, HERNANDO; WITWER, KENNETH; WAUBEN, MARCA; NOLTE-'T HOEN, ESTHER; OLIVIER, MARTIN; et al. Highlights of the Sao Paulo ISEV workshop on extracellular vesicles in cross-kingdom communication. JOURNAL OF EXTRACELLULAR VESICLES, v. 6, n. 1, NOV 26 2017. (16/17245-4, 16/01917-3, 16/12111-0)

MADEIRA, RAFAEL PEDRO; DAL'MAS ROMERA, LAVINIA MARIA; DE CASSIA BUCK, PAULA; MADY, CHARLES; IANNI, BARBARA MARIA; TORRECILHAS, ANA CLAUDIA. New Biomarker in Chagas Disease: Extracellular Vesicles Isolated from Peripheral Blood in Chronic Chagas Disease Patients Modulate the Human Immune Response. JOURNAL OF IMMUNOLOGY RESEARCH, v. 2021, JAN 12 2021. (16/01917-3)

LARISSA F PARANAIBA; ALESSANDRA A GUARNERI; ANA C TORRECILHAS; MARIA N MELO; RODRIGO P SOARES. Extracellular vesicles isolated from Trypanosoma cruzi affect early parasite migration in the gut of Rhodnius prolixus but not in Triatoma infestans. Memórias do Instituto Oswaldo Cruz, v. 114, 2019-12-13. (16/01917-3)