Supplementation Paper Assessment with Omega -3 and laser therapy of low intensity in the Thalamus in Experimental Model of Ischemic Stroke

• Grant number: 16/10286-7
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2016
• End date: August 31, 2019
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Regiane Albertini
• Grantee: Regiane Albertini
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Federal de São Paulo (UNIFESP). Campus São José dos Campos. São José dos Campos , SP, Brazil
• Associated researchers: Carla Alessandra Scorza Bahi ; Fulvio Alexandre Scorza

Abstract

The Stroke (CVA) is a sudden neurological condition, which mostly result from acerebral ischemia. Responsible for a large number of deaths and disability worldwide,it is of paramount importance to implement prevention strategies and identify newtherapies. Although they have been described some benefits of using omega-3 (É3)and low intensity laser therapy (LLLT) in stroke, no study has found that repercussionsin the thalamus. The overall objective of the study is to analyze the effects ofsupplementation with É3 and the role of LLLT on the thalamus in an experimentalmodel of stroke Ischemic (AVCI). The stroke will be induced by the method ofphotothrombosis. Will be used 72 male Wistar rats (230 ± 20g) were randomly dividedinto 6 experimental groups with 12 animals, as follows: control group (C), ControlCremophor (CC), Group AVC Omega-3 Prophylactic (AVCP) AVC group Omega-3Therapeutic (AVCT), Omega-3 Prophylactic and Therapeutic group (AVCPT) and AVCgroup Laser Therapy (AVCL). a dose of 85mg / kg / day of fish oil will be administered((PROEPA®) via gavage, and the application of LLLT transcranial will be performed by alaser diode equipment (Photothera) with a wavelength of 808 ± 5nm , 7,5mW powerdensity / cm2. It will be performed histological analysis, immunohistochemistry,enzyme-linked immunosorbent assay (ELISA), electrophysiological study and recordingof cortical and cardiac electrical activity (EEG and ECG). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VOGEL, DEBORA D. S.; ORTIZ-VILLATORO, NANCY N.; ARAUJO, NOEMI S.; MARQUES, MARCIA JONATHAS GUIMARAES; AIMBIRE, FLAVIO; SCORZA, FULVIO A.; SCORZA, CARLA A.; ALBERTINI, REGIANE. Transcranial low-level laser therapy in an in vivo model of stroke: Relevance to the brain infarct, microglia activation and neuroinflammation. Journal of Biophotonics, v. 14, n. 6, MAR 2021. (16/10286-7)

VOGEL, DEBORA D. S.; ORTIZ-VILLATORO, NANCY N.; DE FREITAS, LEANDRO; AIMBIRE, FLAVIO; SCORZA, FULVIO A.; ALBERTINI, REGIANE; SCORZA, CARLA A.. Repetitive transcranial photobiomodulation but not long-term omega-3 intake reduces epileptiform discharges in rats with stroke-induced epilepsy. Journal of Biophotonics, v. 14, n. 1, JAN 2021. (16/10286-7)