The role of ADAM 17-containing exosomes in sculpting metastatic niche in oral cancer

• Grant number: 16/50005-7
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2016
• End date: October 31, 2018
• Field of knowledge: Health Sciences - Dentistry
• Mobility Program: SPRINT - Projetos de pesquisa - Mobilidade
• Principal Investigator: Adriana Franco Paes Leme
• Grantee: Adriana Franco Paes Leme
• Principal researcher abroad: Jay William Fox
• Institution abroad: University of Virginia (UVa), United States
• Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil
• Associated research grant: 10/19278-0 - Study of regulation of ADAMs in oral cancer, AP.JP

Abstract

Considering the development of regional metastasis in OSCC is the main prognostic factor in OSCC, we aim to evaluate the role of ADAM17-overexpressing vesicles in pre-metastatic niche preparation. For that, ADAM17-overexpressing EV from HEK293 Flp-In TREX cells will be isolated, evaluated by Nano particle tracking, Cryo electron microscopy and Mass spectrometry. Male BALB/c nude mice will receive in the pad of the left anterior member 5 injections of label-vesicles originated from HEK293 following by the injection of the SCC-9 cells and/or less aggressive SCC-15 or SCC-25. The lymph nodes will be evaluated by immunofluorescence and histopathological examination. The proteome of vesicles will be examined and the effect of EV on recipient lymph nodes tissue evaluated by Target RNA sequencing. We expect that this study will provide candidate proteins that are potential hubs in oral cancer metastasis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZANDONADI, FLAVIA S.; YOKOO, SAMI; GRANATO, DANIELA CAMPOS; RIVERA, CESAR; SOARES MACEDO, CAROLINA CARNEIRO; SOARES, CIRO DANTAS; CARNIELLI, CAROLINA MORETTO; DOMINGUES, ROMENIA RAMOS; PAULETTI, BIANCA A.; CONSONNI, SILVIO ROBERTO; et al. Follistatin-related protein 1 interacting partner of Syndecan-1 promotes an aggressive phenotype on Oral Squamous cell carcinoma (OSCC) models. JOURNAL OF PROTEOMICS, v. 254, p. 13-pg., 2022-03-15. (13/02257-9, 18/18496-6, 16/50005-7, 18/15535-0, 16/07846-0, 19/18751-9, 09/54067-3, 10/19278-0)

BUSSO-LOPES, ARIANE F.; CARNIELLI, CAROLINA M.; WINCK, FLAVIA; RIANO-PACHON, DIEGO M.; OLIVEIRA, ANA K.; COSTA, RUTE A.; RIVERA, CESAR; CALDANA, CAMILA; FOX, JAY W.; PAES LEME, ADRIANA F.. Multi-omics data indicate that primary and lymph node oral cancer cells-derived extracellular vesicles carry cargo molecules with a specific aggressive pattern. Cancer Research, v. 78, n. 13, p. 2-pg., 2018-07-01. (16/50005-7)