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Cadmium transport and accumulation of different tomato genotypes: physiological, proteomic and molecular approaches

Grant number: 16/14349-3
Support type:Regular Research Grants
Duration: December 01, 2016 - November 30, 2018
Field of knowledge:Agronomical Sciences - Agronomy
Principal Investigator:Ricardo Antunes de Azevedo
Grantee:Ricardo Antunes de Azevedo
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Assoc. researchers:Berenice Kussumoto de Alcântara da Silva ; Fernanda Salvato ; Fernando Angelo Piotto ; Katherine Derlene Batagin Piotto ; Salete Aparecida Gaziola

Abstract

In the last decades, agriculture has been changed with arising of new scenarios of environmental problems, especially those involving the contamination of soil and water with heavy metals. These problems are more serious when we consider that metals such as cadmium (Cd) can easily enter in food chain by their absorption via the roots of the plant species used as foods. Although several studies have been conducted to understand the Cd impact on oxidative metabolism of crops, there are little information about the kinetics of uptake and accumulation of this metal in the tissues, as well as on dynamic of responses in biochemical and molecular levels throughout the process. This information is extremely important because they can ravel mechanisms response to Cd and mechanisms of tolerance in an integrated and complex system. Then, objective of this work is characterize the physiological, biochemical and molecular changes during the process of absorption and Cd accumulation, through the integrated analysis of all these processes using sensitive tomato genotypes to this metal. We hope to draw a map in which the processes of absorption and Cd accumulation in tomato plants are monitored from the first moment in which the plants come into contact with the metal until the time that they are under chronic stress. Associated with these kinetic studies of absorption and Cd accumulation, we intend to trace the cellular antioxidant system response profile, in addition to conducting the study on protein change, phytochelatin synthesis and molecular level of expression of genes related to the transport of ions on solution to the plant via root tissues. For that, we will apply methodology of analysis of Cd uptake kinetics and nutrients over time, conducting experiments with plants in hydroponics system, to identify the most important times of recognition and response to stress. In addition, we will use reciprocal grafting between tolerant and susceptible tomato genotypes, as tools to study the signaling between the root system and aerial parts of the plants during the process of absorption and metal accumulation in tissues. These results and the previous knowledge of other researches involving the response of crops to Cd exposure, aggregating the experience of our research group, certainly will generate relevant information, which will be published in good refereed international journals that are respected in the field. (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CARVALHO, MARCIA E. A.; GAZIOLA, SALETE A.; CARVALHO, LILIAN A.; AZEVEDO, RICARDO A. Cadmium effects on plant reproductive organs: Physiological, productive, evolutionary and ecological aspects. Annals of Applied Biology, JUN 2020. Web of Science Citations: 0.
CARVALHO, MARCIA E. A.; CASTRO, PAULO R. C.; KOZAK, MARCIN; AZEVEDO, RICARDO A. The sweet side of misbalanced nutrients in cadmium-stressed plants. Annals of Applied Biology, v. 176, n. 3 JAN 2020. Web of Science Citations: 0.
REIS BORGES, KARINA LIMA; SALVATO, FERNANDA; LOZIUK, PHILIP L.; MUDDIMAN, DAVID C.; AZEVEDO, RICARDO ANTUNES. Quantitative proteomic analysis of tomato genotypes with differential cadmium tolerance. Environmental Science and Pollution Research, v. 26, n. 25, p. 26039-26051, SEP 2019. Web of Science Citations: 0.
REIS BORGES, KARINA LIMA; RIEGER HIPPLER, FRANZ WALTER; AMARAL CARVALHO, MARCIA EUGENIA; NALIN, RAFAEL STORTO; MATIAS, FILIPE INACIO; AZEVEDO, RICARDO ANTUNES. Nutritional status and root morphology of tomato under Cd-induced stress: Comparing contrasting genotypes for metal-tolerance. Scientia Horticulturae, v. 246, p. 518-527, FEB 27 2019. Web of Science Citations: 4.
REIS BORGES, KARINA LIMA; SALVATO, FERNANDA; ALCANTARA, BERENICE KUSSUMOTO; NALIN, RAFAEL STORTO; PIOTTO, FERNANDO ANGELO; AZEVEDO, RICARDO ANTUNES. Temporal dynamic responses of roots in contrasting tomato genotypes to cadmium tolerance. ECOTOXICOLOGY, v. 27, n. 3, p. 245-258, APR 2018. Web of Science Citations: 19.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.