From stress to embryos: molecular and physiological mechanisms associated to somatic embryogenesis

• Grant number: 16/17541-2
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2017
• End date: July 31, 2019
• Field of knowledge: Biological Sciences - Botany - Applied Botany
• Principal Investigator: Eny Iochevet Segal Floh
• Grantee: Eny Iochevet Segal Floh
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Associated researchers: André Luiz Wendt dos Santos ; Diego Trindade de Souza ; Marcos Silveira Buckeridge ; Paula Maria Elbl
• Associated scholarship(s): 18/23381-3 - Auxin, reactive oxygen species and nitric oxide during the development of somatic cells in Araucaria angustifolia (Bertol.) Kuntze, BP.TT; 18/12261-7 - Auxin, reactive oxygen species and nitric oxide during the development of somatic cells in Araucaria angustifolia (Bertol.) Kuntze, BP.TT

Abstract

The aim of this project is to identify molecular and physiological mechanisms provoked by stress during in vitro embryo development in Brazilian pine. To achieve this aim, the project will use information generated from Brazilian pine transcriptome (FAPESP proc.2011/51659-7) and cell signaling during morphogenesis (FAPESP proc. 2014/11228-5) with physiological, biochemical and molecular analysis of cell lines with different degrees of embryogenicity and during transdifferentiation of proembryogenic masses into somatic embryos. For the identification of molecular and physiological mechanisms associated to stressful conditions during somatic embryo formation, we will characterize the metabolism of amino acids, polyamines and carbohydrates, the emission of reactive oxygen/nitrogen species and their impact on auxin homeostasis and post-translational modifications promoted by S-nitrosylation. The results will bring new insights on basic and applied plant biology, namely in stress signaling pathways active during conifer embryo development, as well as in the ability to use somatic embryogenesis to propagate Brazilian pine genotypes. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NAVARRO, V, BRUNO; ELBL, PAULA; DE OLIVEIRA, LEANDRO F.; PIOVEZANI, AMANDA R.; DOS SANTOS, ANDRE L. W.; DE SOUZA, DIEGO T.; DEMARCO, DIEGO; BUCKERIDGE, MARCOS S.; FLOH, ENY I. S.. Cell-to-cell trafficking patterns in cell lines of Araucariaangustifolia (Brazilian pine) with contrasting embryogenic potential. PLANT CELL TISSUE AND ORGAN CULTURE, v. 148, n. 1, SEP 2021. (16/17541-2, 14/50884-5, 14/26888-0)

NAVARRO, BRUNO V.; DE OLIVEIRA, LEANDRO F.; DE OLIVEIRA, LAUANA P.; ELBL, PAULA; MACEDO, AMANDA F.; BUCKERIDGE, MARCOS S.; FLOH, ENY I. S.. Starch turnover is stimulated by nitric oxide in embryogenic cultures of Araucaria angustifolia. PLANT CELL TISSUE AND ORGAN CULTURE, v. 147, n. 3, AUG 2021. (14/11228-5, 14/26888-0, 16/17541-2)

SIMOES, MARCELLA SIQUEIRA; FERREIRA, SAVIO SIQUEIRA; GRANDIS, ADRIANA; RENCORET, JORGE; PERSSON, STAFFAN; FLOH, ENY IOCHEVET SEGAL; FERRAZ, ANDRE; DEL RIO, JOSE C.; BUCKERIDGE, MARCOS SILVEIRA; CESARINO, IGOR. Differentiation of Tracheary Elements in Sugarcane Suspension Cells Involves Changes in Secondary Wall Deposition and Extensive Transcriptional Reprogramming. FRONTIERS IN PLANT SCIENCE, v. 11, DEC 18 2020. (14/50884-5, 16/50189-0, 15/02527-1, 19/13936-0, 15/18361-5, 16/06917-1, 16/17541-2)

DE OLIVEIRA, LEANDRO FRANCISCO; DOS SANTOS, ANDRE LUIS WENDT; FLOH, ENY IOCHEVET SEGAL. Polyamine and amino acid profiles in immature Araucaria angustifolia seeds and their association with embryogenic culture establishment. TREES-STRUCTURE AND FUNCTION, v. 34, n. 3, DEC 2019. (15/21075-4, 12/22738-9, 16/17541-2, 17/01284-3, 18/08215-0)

ELBL, PAULA M.; DE SOUZA, DIEGO T.; ROSADO, DANIELE; DE OLIVEIRA, LEANDRO F.; NAVARRO, V, BRUNO; MATIOLI, SERGIO R.; FLOH, ENY I. S.. Building an embryo: An auxin gene toolkit for zygotic and somatic embryogenesis in Brazilian pin. Gene, v. 817, APR 5 2022. (18/08215-0, 16/17541-2, 17/25680-5)