Use of PGF as a tool for predictability and success of pacu induced spawning

• Grant number: 16/20741-3
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2017
• End date: March 31, 2019
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Sergio Ricardo Batlouni
• Grantee: Sergio Ricardo Batlouni
• Host Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated researchers: Rafael Yutaka Kuradomi

Abstract

In this proposal, we intend to evaluate the effect (first aim) and set the time of application (second aim) of prostaglandin F (PGF) on hormonal induction of pacu spawning by hypophysation. For the first objective, 48 females will be randomly distributed among four treatments: EBHC (crude extract of carp pituitary gland), EBHC + PGF; PGF; and control (saline). Each fish (1 per box) will be considered as an experimental unit and will be monitored over time. A completely randomized design (CRD) with a longitudinal factor will be used: four treatments, 12 repetitions, evaluated at five times (4x12x5). At the end of the experiment, females will be classified by presenting or not successful spawning. The average fecundity, fertility and hatching rates, as well as the profiles of plasma and ovarian concentrations of PGE, PGF, gonadal steroids and gene expression of steroidogenic enzymes over time will be determined and compared among different treatments. For the second aim, the efficacy of using three different times of PGF application between the second hormonal dose and ovulation will be evaluated. Each fish will be considered as an experimental unit in a CRD: five groups (three treatments and two controls), 10 repetitions, assessed at the time of ovulation (5x10). Females will be classified as a spawned or not spawned. The ovulation rates, fecundity fertility and hatch, as well as plasma levels of PGE, PGF, gonadal steroids in time of ovulation will be determined and compared among groups. In this study, we aim to generate basic and specialized knowledge upon the effect of exogenous PGF on pacu ovulation (first goal), more specifically, assess whether treatments that propitiate the highest values of endogenous PGF and / or DHP, at the time spawning, will be those that present higher rates of ovulation. Finally, we intend to determine the time of exogenous PGF application that provides the best ovulation rates (second goal) generating a new protocol with greater predictability of success. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SATO, RAFAEL TOMODA; KURADOMI, RAFAEL YUTAKA; CALIL, MARINA CABRAL; DE JESUS SILVA, LAIZA MARIA; DE ABREU, MARIANA ROZA; FIGUEIREDO-ARIKI, DANIEL GUIMARAES; DE FREITAS, GUILHERME ANTONIO; BATLOUNI, SERGIO RICARDO. Resumption and progression of meiosis and circulating levels of steroids and prostaglandin F-2 alpha of Piaractus mesopotamicus induced by hypophysation with prostaglandin F-2 alpha. AQUACULTURE RESEARCH, v. 52, n. 3, NOV 2020. (16/20741-3)