Study the chemical composition of fungi and plants of the Atlantic Forest aimed at antitumor, antiparasitic and antimicrobial potential

• Grant number: 16/24985-4
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2017
• End date: March 31, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Patricia Sartorelli
• Grantee: Patricia Sartorelli
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated researchers: André Gustavo Tempone Cardoso ; Cassius Vinicius Stevani ; João Henrique Ghilardi Lago ; Luiz Felipe Domingues Passero ; Marcelo Afonso Vallim ; Marcelo José Pena Ferreira ; Renata Castiglioni Pascon

Abstract

The present project aims to evaluate the antimicrobial, antitumor and antiparasitary potential of plant species, endophytic fungi associated with these species and basidiomycete fungi that will be collected in the Atlantic Forest of the state of São Paulo aiming at the isolation of special bioactive metabolites. In this way, species belonging to the various plant families, associated endophytic fungi and basidiomycete fungi will be collected and their extracts will be prepared and then submitted to a sequence of bioassays, in order to select those that present potential biological activity, mainly antimicrobial action (antifungal and bactericidal) , antiparasitic (antileishmanial and anti-Chagas) and antitumor. Next, the promising extracts will be fractionated, the active metabolites purified by chromatographic techniques and the respective structures determined by spectroscopic and spectrometric techniques. In addition, the essential oils of the plant species will be obtained, which will be analyzed chemically and submitted to the evaluation of the biological potential. After identification of the active substances, they can be submitted to specific tests to define the mode of action of the active substances. In this way, the present project intends to add new compounds and / or extracts with proven pharmacological activity to the restricted phytotherapeutic therapeutic arsenal, adding value to plant or fungal species as well as the metabolites from the remaining areas of the Atlantic Forest of the State of São Paulo, aiming at Preservation of them. (AU)

Scientific publications

(17)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CALDAS, LHAIS ARAUJO; HORVATH, RENATO O.; FERREIRA-SILVA, GUILHERME ALVARO; FERREIRA, MARCELO J. P.; IONTA, MARISA; SARTORELLI, PATRICIA. Calein C, a Sesquiterpene Lactone Isolated From Calea Pinnatifida (Asteraceae), Inhibits Mitotic Progression and Induces Apoptosis in MCF-7 Cells. FRONTIERS IN PHARMACOLOGY, v. 9, OCT 18 2018. (16/24985-4)

SANTOS, AUGUSTO L.; SOARES, MARISI G.; DE MEDEIROS, LIVIA S.; FERREIRA, MARCELO J. P.; SARTORELLI, PATRICIA. Identification of flavonoid-3-O-glycosides from leaves of Casearia arborea (Salicaceae) by UHPLC-DAD-ESI-HRMS/MS combined with molecular networking and NMR. Phytochemical Analysis, FEB 2021. (14/21593-2, 16/24985-4)

CALDAS, LHAIS A.; SOARES, DOUGLAS M. M.; MENOLLI, NELSON JR JR; STEVANI, V, CASSIUS; SARTORELLI, PATRICIA. etabolomics of the wild mushroom Gymnopilus imperialis (Agaricomycetes, Basidiomycota) by UHPLC-HRMS/MS analysis and molecular networ. FUNGAL BIOLOGY, v. 126, n. 2, p. 132-138, FEB 2022. (18/15677-0, 17/22501-2, 16/24985-4, 19/12605-0, 18/25646-4)

CALDAS, LHAIS ARAUJO; ZIED, DIEGO CUNHA; SARTORELLI, PATRICIA. Dereplication of extracts from nutraceutical mushrooms Pleurotus using molecular network approach. Food Chemistry, v. 370, FEB 15 2022. (16/24985-4, 18/21492-2, 18/25646-4)

CALDAS, LHAIS ARAUJO; YOSHINAGA, MEIRE L.; FERREIRA, MARCELO J. P.; LAGO, JOAO H. G.; DE SOUZA, ADRIANA B.; LAURENTI, MARCIA D.; PASSERO, LUIZ FELIPE D.; SARTORELLI, PATRICIA. Antileishmanial activity and ultrastructural changes of sesquiterpene lactones isolated from Calea pinnatifida (Asteraceae). BIOORGANIC CHEMISTRY, v. 83, p. 348-353, MAR 2019. (16/24985-4, 16/00468-0)

SANTOS, AUGUSTO L.; IONTA, MARISA; HORVATH, RENATO; SOARES, MARISI G.; MEDEIROS, LIVIA S. DE; UEMI, MIRIAM; KAWAFUNE, EUNIZINIS S.; TANGERINA, MARCELO M. P.; FERREIRA, MARCELO J. P.; SARTORELLI, PATRICIA. Molecular network for accessing polyketide derivatives from Phomopsis sp., an endophytic fungus of Casearia arborea (Salicaceae). PHYTOCHEMISTRY LETTERS, v. 42, p. 1-7, APR 2021. (14/21593-2, 16/24985-4, 17/16606-6)

CALDAS, LHAIS ARAUJO; HORVATH, RENATO O.; FERREIRA-SILVA, GUILHERME ALVARO; FERREIRA, MARCELO J. P.; IONTA, MARISA; SARTORELLI, PATRICIA. Calein C, a Sesquiterpene Lactone Isolated From Calea Pinnatifida (Asteraceae), Inhibits Mitotic Progression and Induces Apoptosis in MCF-7 Cells. FRONTIERS IN PHARMACOLOGY, v. 9, p. 10-pg., 2018-10-18. (16/24985-4)

CALDAS, LHAIS A.; SANTOS, PATRICIA D.; CARBONERO, ELAINE R.; IONTA, MARISA; MIYAZAWA, MARTA; CAIXETA, ESTER S.; FREGNAN, ANTONIO M.; NOBREGA, BIANCA BARROS; DI MEDEIROS, MARIA CAROLINA B.; MENOLLI JR, NELSON; et al. Immunomodulatory Effect of Polysaccharides from the Mushroom-Forming Basidiomycete Gymnopilus imperialis (Agaricomycetes, Basidiomycota). PHARMACEUTICALS, v. 15, n. 10, p. 13-pg., 2022-10-01. (22/09202-4, 18/15677-0, 16/24985-4, 19/12605-0, 17/22501-2, 18/25646-4)

CALDAS, LHAIS ARAUJO; RODRIGUES, MARIANA T.; BATISTA, ANDREA N. L.; BATISTA, JR., JOAO M.; LAGO, JOAO H. G.; FERREIRA, MARCELO J. P.; RUBIO, ILEANA G. S.; SARTORELLI, PATRICIA. Sesquiterpene Lactones from Calea pinnatifida: Absolute Configuration and Structural Requirements for Antitumor Activity. Molecules, v. 25, n. 13, JUL 2020. (16/24985-4, 14/25222-9)

SANTOS, AUGUSTO L.; SOARES, MARISI G.; DE MEDEIROS, LIVIA S.; FERREIRA, MARCELO J. P.; SARTORELLI, PATRICIA. Identification of flavonoid-3-O-glycosides from leaves of Casearia arborea (Salicaceae) by UHPLC-DAD-ESI-HRMS/MS combined with molecular networking and NMR. Phytochemical Analysis, v. 32, n. 6, p. 891-898, NOV 2021. (16/24985-4, 14/21593-2)

SANTOS, AUGUSTO L.; IONTA, MARISA; HORVATH, RENATO O.; SOARES, MARISI G.; SILVA, DANIELE O.; KAWAFUNE, EUNIZINIS S.; FERREIRA, MARCELO J. P.; SARTORELLI, PATRICIA. Dereplication of Cytochalasans and Octaketides in Cytotoxic Extracts of Endophytic Fungi from Casearia arborea (Salicaceae). METABOLITES, v. 12, n. 10, p. 12-pg., 2022-10-01. (20/16554-9, 16/24985-4)

ERICK P. DANTAS; JACKSON MONTEIRO; LÍVIA S. DE MEDEIROS; MAIARA M. ROMANELLI; MAIARA AMARAL; ANDRE G. TEMPONE; JOÃO HENRIQUE G. LAGO; MARISI G. SOARES; PATRICIA SARTORELLI. Dereplication of Aporphine Alkaloids by UHPLC-HR-ESI-MS/MS and NMR from Duguetia lanceolata St. -Hil (Annonaceae) and Antiparasitic Activity Evaluation. Journal of the Brazilian Chemical Society, v. 31, n. 9, p. 1908-1916, 2020-08-19. (18/10279-6, 16/24985-4)

MONTEIRO, JACKSON; PASSERO, LUIZ FELIPE D.; JESUS, JESSICA A.; LAURENTI, MARCIA D.; LAGO, JOAO H. G.; SOARES, MARISI G.; BATISTA, ANDREA N. L.; BATISTA JR, JOAO M.; SARTORELLI, PATRICIA. Absolute Configuration and Antileishmanial Activity of (-)-Cyclocolorenone Isolated from Duguetia lanceolata (Annonaceae). CURRENT TOPICS IN MEDICINAL CHEMISTRY, v. 22, n. 19, p. 8-pg., 2022-01-01. (18/24077-6, 21/02789-7, 19/22319-5, 16/24985-4)

ALEXANDRE, TATIANA RODRIGUES; LIMA, MARTA LOPES; GALUPPO, MARIANA KOLOS; MESQUITA, JULIANA TONINI; DO NASCIMENTO, MATILIA ANA; DOS SANTOS, AUGUSTO LEONARDO; SARTORELLI, PATRICIA; PIMENTA, DANIEL CARVALHO; TEMPONE, ANDRE GUSTAVO. Ergosterol isolated from the basidiomycete Pleurotus salmoneostramineus affects Trypanosoma cruzi plasma membrane and mitochondria. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 23, n. 1, MAY 30 2017. (16/24985-4, 13/50228-8)

ARAUJO, CALDAS LHAIS; CUNHA, ZIED DIEGO; PATRICIA, SARTORELLI. Dereplication of extracts from nutraceutical mushrooms Pleurotus using molecular network approach. Planta Medica, v. 87, n. 15, p. 1-pg., 2021-12-01. (18/21492-2, 16/24985-4, 18/25646-4)

DOS SANTOS, AUGUSTO L. L.; AMARAL, MAIARA; HASEGAWA, FLAVIA RIE; LAGO, JOAO HENRIQUE G.; TEMPONE, ANDRE G.; SARTORELLI, PATRICIA. (-)-T-Cadinol, a Sesquiterpene Isolated From Casearia sylvestris (Salicaceae)-Displayed In Vitro Activity and Causes Hyperpolarization of the Membrane Potential of Trypanosoma cruzi (vol 12, 734127, 2021). FRONTIERS IN PHARMACOLOGY, v. 13, p. 1-pg., 2022-03-14. (16/24985-4, 18/10279-6, 21/02789-7)

DANTAS, ERICK P.; MONTEIRO, JACKSON; DE MEDEIROS, LIVIA S.; ROMANELLI, MAIARA M.; AMARAL, MAIARA; TEMPONE, ANDRE G.; LAGO, JOAO HENRIQUE G.; SOARES, MARISI G.; SARTORELLI, PATRICIA. Dereplication of Aporphine Alkaloids by UHPLC-HR-ESI-MS/MS and NMR from Duguetia lanceolata St.-Hit (Annonaceae) and Antiparasitic Activity Evaluation. Journal of the Brazilian Chemical Society, v. 31, n. 9, p. 9-pg., 2020-09-01. (16/24985-4, 18/10279-6)